Chromatin reassembly signals the end of DNA repair

Chin Chuan Chen, Jessica Tyler

    Research output: Contribution to journalReview article

    23 Scopus citations

    Abstract

    The packaging of the eukaryotic genome into chromatin provides a formidable obstacle to the machinery that mediates genomic processes such as transcription, repair and replication. The cell solves this accessibility problem during double-strand DNA repair by removing the histone proteins flanking the DNA lesion. Presumably then, the repaired DNA is subsequently reassembled into chromatin in order to allow the epigenetic information to be restored and to repackage and protect the genome. Our recent work has shown that chromatin is indeed reassembled following double-strand break repair in budding yeast. Furthermore, the assembly of the repaired DNA into chromatin is driven by acetylation within the globular domain of histone H3, on lysine 56 (H3 K56Ac). Unexpectedly, we also discovered that H3 K56Ac and chromatin assembly onto the repaired DNA is essential to turn off the DNA damage cell cycle checkpoint. This work demonstrates that reformation of the chromatin structure, not DNA repair per se, is the elusive signal that tells the cell when DNA repair is complete.

    Original languageEnglish (US)
    Pages (from-to)3792-3797
    Number of pages6
    JournalCell Cycle
    Volume7
    Issue number24
    DOIs
    StatePublished - Dec 15 2008

    Keywords

    • Checkpoint
    • Chromatin
    • Histone acetylation
    • Histone chaperones
    • Recovery

    ASJC Scopus subject areas

    • Molecular Biology
    • Developmental Biology
    • Cell Biology

    Fingerprint Dive into the research topics of 'Chromatin reassembly signals the end of DNA repair'. Together they form a unique fingerprint.

  • Cite this