TY - JOUR
T1 - Chromosome 18 suppresses the tumorigenicity of prostate cancer cells
AU - Padalecki, Susan S.
AU - Johnson-Pais, Teresa L.
AU - Killary, Ann M.
AU - Leach, Robin J.
PY - 2001
Y1 - 2001
N2 - Microcell-mediated chromosome transfer allows for the introduction of normal chromosomes into tumor cells in an effort to identify putative tumor suppressor genes. We have used this approach to introduce an intact copy of chromosome 18 into the prostate cancer cell line DUI45, and independently to introduce human chromosomes 8 and 18 into the prostate cancer cell line TSU-PRI. Introduction of an extra copy of human chromosome 8 had no effect on the growth properties in vitro or the tumorigenicity in vivo of TSU-PRI cells. However, microcell hybrids containing an introduced copy of human chromosome 18 exhibited a longer population doubling time, retarded growth in soft agar, and slowed tumor growth in athymic nude mice. These experiments provide functional evidence for the presence of one or more tumor suppressor genes on human chromosome 18 that are involved in prostate cancer.
AB - Microcell-mediated chromosome transfer allows for the introduction of normal chromosomes into tumor cells in an effort to identify putative tumor suppressor genes. We have used this approach to introduce an intact copy of chromosome 18 into the prostate cancer cell line DUI45, and independently to introduce human chromosomes 8 and 18 into the prostate cancer cell line TSU-PRI. Introduction of an extra copy of human chromosome 8 had no effect on the growth properties in vitro or the tumorigenicity in vivo of TSU-PRI cells. However, microcell hybrids containing an introduced copy of human chromosome 18 exhibited a longer population doubling time, retarded growth in soft agar, and slowed tumor growth in athymic nude mice. These experiments provide functional evidence for the presence of one or more tumor suppressor genes on human chromosome 18 that are involved in prostate cancer.
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U2 - 10.1002/1098-2264(2000)9999:9999<::AID-GCC1093>3.0.CO;2-L
DO - 10.1002/1098-2264(2000)9999:9999<::AID-GCC1093>3.0.CO;2-L
M3 - Article
C2 - 11170278
AN - SCOPUS:0035147019
SN - 1045-2257
VL - 30
SP - 221
EP - 229
JO - Genes Chromosomes and Cancer
JF - Genes Chromosomes and Cancer
IS - 3
ER -