TY - JOUR
T1 - Chronic cigarette smoking alters erythrocyte membrane lipid composition and properties in male human volunteers
AU - Padmavathi, Pannuru
AU - Reddy, Vaddi Damodara
AU - Kavitha, Godugu
AU - Paramahamsa, Maturu
AU - Varadacharyulu, Nallanchakravarthula
N1 - Funding Information:
We thank University Grants Commission (UGC), New Delhi, India for providing Rajiv Gandhi National Research Fellowship to P. Padmavathi. The authors also thank Director and Staff of the Center for Cellular and Molecular Biology, Hyderabad, India where fluidity studies were carried out.
PY - 2010/11
Y1 - 2010/11
N2 - Cigarette smoking is a major lifestyle factor influencing the health of human beings. The present study investigates smoking induced alterations on the erythrocyte membrane lipid composition, fluidity and the role of nitric oxide. Thirty experimental and control subjects (age 35±8) were selected for the study. Experimental subjects smoke 12±2 cigarettes per day for 7-10years. In smokers elevated nitrite/nitrate levels in plasma and red cell lysates were observed. Smokers showed increased hemolysis, erythrocyte membrane lipid peroxidation, protein carbonyls, C/P ratio (cholesterol and phospholipid ratio), anisotropic (γ) value with decreased Na+/K+-ATPase activity and sulfhydryl groups. Alterations in smokers erythrocyte membrane individual phospholipids were also evident from the study. Red cell lysate nitric oxide positively correlated with C/P ratio (r=0.565) and fluorescent anisotropic (γ) value (r=0.386) in smokers. Smoking induced generation of reactive oxygen/nitrogen species might have altered erythrocyte membrane physico-chemical properties.
AB - Cigarette smoking is a major lifestyle factor influencing the health of human beings. The present study investigates smoking induced alterations on the erythrocyte membrane lipid composition, fluidity and the role of nitric oxide. Thirty experimental and control subjects (age 35±8) were selected for the study. Experimental subjects smoke 12±2 cigarettes per day for 7-10years. In smokers elevated nitrite/nitrate levels in plasma and red cell lysates were observed. Smokers showed increased hemolysis, erythrocyte membrane lipid peroxidation, protein carbonyls, C/P ratio (cholesterol and phospholipid ratio), anisotropic (γ) value with decreased Na+/K+-ATPase activity and sulfhydryl groups. Alterations in smokers erythrocyte membrane individual phospholipids were also evident from the study. Red cell lysate nitric oxide positively correlated with C/P ratio (r=0.565) and fluorescent anisotropic (γ) value (r=0.386) in smokers. Smoking induced generation of reactive oxygen/nitrogen species might have altered erythrocyte membrane physico-chemical properties.
KW - Cigarette smoking
KW - Erythrocyte membrane
KW - Membrane fluidity
KW - Na/K-ATPase
KW - Nitric oxide
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U2 - 10.1016/j.niox.2010.05.287
DO - 10.1016/j.niox.2010.05.287
M3 - Article
C2 - 20561918
AN - SCOPUS:77956011362
SN - 1089-8603
VL - 23
SP - 181
EP - 186
JO - Nitric Oxide - Biology and Chemistry
JF - Nitric Oxide - Biology and Chemistry
IS - 3
ER -