Clonal Analysis Using Recombinant DNA Probes from the X-Chromosome

Bert Vogelstein; Eric R. Fearon; Stanley R. Hamilton; Ann C. Preisinger; Huntington F. Willard; Alan M. Michelson; Arthur D. Riggs; Stuart H. Orkin

Clonal Analysis Using Recombinant DNA Probes from the X-Chromosome

Bert Vogelstein, Eric R. Fearon, Stanley R. Hamilton, Ann C. Preisinger, Huntington F. Willard, Alan M. Michelson, Arthur D. Riggs, Stuart H. Orkin

Graduate School of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

425 Scopus citations

Abstract

It has been demonstrated that restriction fragment length polymorphisms of X-chromosome genes can be used in conjunction with methylation patterns to determine the clonal composition of human tumors. In this report, we show that several X-chromosome probes can be used for such analyses. In particular, probes derived from the hypoxanthine phosphoribosyltransferase gene and the phosphoglycerate kinase gene could be used for clonal analysis in over 50% of American females. The X-inactivation patterns observed with these probes were found to accurately reflect clonality in more than 95% of 92 tumors tested.

Original language	English (US)
Pages (from-to)	4806-4813
Number of pages	8
Journal	Cancer Research
Volume	47
Issue number	18
State	Published - 1987

ASJC Scopus subject areas

Oncology
Cancer Research

Cite this

@article{652029b018c44347964069fefc00eeba,

title = "Clonal Analysis Using Recombinant DNA Probes from the X-Chromosome",

abstract = "It has been demonstrated that restriction fragment length polymorphisms of X-chromosome genes can be used in conjunction with methylation patterns to determine the clonal composition of human tumors. In this report, we show that several X-chromosome probes can be used for such analyses. In particular, probes derived from the hypoxanthine phosphoribosyltransferase gene and the phosphoglycerate kinase gene could be used for clonal analysis in over 50% of American females. The X-inactivation patterns observed with these probes were found to accurately reflect clonality in more than 95% of 92 tumors tested.",

author = "Bert Vogelstein and Fearon, {Eric R.} and Hamilton, {Stanley R.} and Preisinger, {Ann C.} and Willard, {Huntington F.} and Michelson, {Alan M.} and Riggs, {Arthur D.} and Orkin, {Stuart H.}",

year = "1987",

language = "English (US)",

volume = "47",

pages = "4806--4813",

journal = "Cancer Research",

issn = "0008-5472",

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T1 - Clonal Analysis Using Recombinant DNA Probes from the X-Chromosome

AU - Vogelstein, Bert

AU - Fearon, Eric R.

AU - Hamilton, Stanley R.

AU - Preisinger, Ann C.

AU - Willard, Huntington F.

AU - Michelson, Alan M.

AU - Riggs, Arthur D.

AU - Orkin, Stuart H.

PY - 1987

Y1 - 1987

N2 - It has been demonstrated that restriction fragment length polymorphisms of X-chromosome genes can be used in conjunction with methylation patterns to determine the clonal composition of human tumors. In this report, we show that several X-chromosome probes can be used for such analyses. In particular, probes derived from the hypoxanthine phosphoribosyltransferase gene and the phosphoglycerate kinase gene could be used for clonal analysis in over 50% of American females. The X-inactivation patterns observed with these probes were found to accurately reflect clonality in more than 95% of 92 tumors tested.

AB - It has been demonstrated that restriction fragment length polymorphisms of X-chromosome genes can be used in conjunction with methylation patterns to determine the clonal composition of human tumors. In this report, we show that several X-chromosome probes can be used for such analyses. In particular, probes derived from the hypoxanthine phosphoribosyltransferase gene and the phosphoglycerate kinase gene could be used for clonal analysis in over 50% of American females. The X-inactivation patterns observed with these probes were found to accurately reflect clonality in more than 95% of 92 tumors tested.

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M3 - Article

C2 - 2887283

AN - SCOPUS:0023626415

SN - 0008-5472

VL - 47

SP - 4806

EP - 4813

JO - Cancer Research

JF - Cancer Research

IS - 18

ER -

Clonal Analysis Using Recombinant DNA Probes from the X-Chromosome

Abstract

ASJC Scopus subject areas

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