TY - JOUR
T1 - Clones derived from human natural killer cells are susceptible to lysis by human interleukin-2-activated killer cells
AU - Fujiwara, T.
AU - Loudon, W. G.
AU - Grimm, E. A.
PY - 1993
Y1 - 1993
N2 - Peripheral blood lymphocytes cultured with interleukin-2 (IL-2), which are referred to as lymphokine-activated killer (LAK) cells, develop the ability to lyse a wide variety of tumor cells but not normal cells. However, we report here that a cloned human natural killer cell line, NK3.3, was susceptible to lysis by human LAK cells. An IL-4-dependent NK3.3 subclone showed higher sensitivity to LAK-mediated lysis than the conditioned medium- dependent parent clone or the IL-2-dependent NK3.3 subclone. Cytokine- 'starved' NK3.3 cells, which were cultured in medium lacking IL-2 or IL-4, exhibited a decreased susceptibility to LAK cell lysis. Periodate treatment of NK3.3 cells, which can induce nonspecific binding between effector cells and target cells through Schiff-base formation, enhanced the sensitivity to LAK, suggesting that adhesion molecules may be involved in this killing mechanism. The 'starved' NK3.3 cells expressed lower levels of intracellular adhesion molecule 1 (ICAM-1); however, anti-ICAM-1 antibody could not inhibit the sensitivity of the NK3.3 clone to LAK lysis. These results suggest that ICAM-1 is not the major ligand in NK3.3 sensitivity, although the sensitivity correlates well with the magnitude of ICAM-1 expression.
AB - Peripheral blood lymphocytes cultured with interleukin-2 (IL-2), which are referred to as lymphokine-activated killer (LAK) cells, develop the ability to lyse a wide variety of tumor cells but not normal cells. However, we report here that a cloned human natural killer cell line, NK3.3, was susceptible to lysis by human LAK cells. An IL-4-dependent NK3.3 subclone showed higher sensitivity to LAK-mediated lysis than the conditioned medium- dependent parent clone or the IL-2-dependent NK3.3 subclone. Cytokine- 'starved' NK3.3 cells, which were cultured in medium lacking IL-2 or IL-4, exhibited a decreased susceptibility to LAK cell lysis. Periodate treatment of NK3.3 cells, which can induce nonspecific binding between effector cells and target cells through Schiff-base formation, enhanced the sensitivity to LAK, suggesting that adhesion molecules may be involved in this killing mechanism. The 'starved' NK3.3 cells expressed lower levels of intracellular adhesion molecule 1 (ICAM-1); however, anti-ICAM-1 antibody could not inhibit the sensitivity of the NK3.3 clone to LAK lysis. These results suggest that ICAM-1 is not the major ligand in NK3.3 sensitivity, although the sensitivity correlates well with the magnitude of ICAM-1 expression.
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M3 - Article
C2 - 8096153
AN - SCOPUS:0027407770
SN - 0277-6766
VL - 12
SP - 45
EP - 49
JO - Lymphokine and Cytokine Research
JF - Lymphokine and Cytokine Research
IS - 1
ER -