Cloning and characterization of a novel member of human β-1,4-galactosyltransferase gene family

Yuxin Fan; Long Yu; Qi Zhang; Ying Jiang; Fangyan Dai; Chiyuan Chen; Qiang Tu; Anding Bi; Yuefang Xu; Shouyuan Zhao

doi:10.1007/bf02882052

Cloning and characterization of a novel member of human β-1,4-galactosyltransferase gene family

Yuxin Fan, Long Yu, Qi Zhang, Ying Jiang, Fangyan Dai, Chiyuan Chen, Qiang Tu, Anding Bi, Yuefang Xu, Shouyuan Zhao

Experimental Radiation Oncology

Research output: Contribution to journal › Article › peer-review

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Abstract

By using the EST strategy for identifying novel members belonging to homologous gene families, a novel full-length cDNA encoding a protein significantly homologous to UDP-Gal: N-acetylglucosamine β-1,4-galactosyltransferase (GalT) was isolated from a human testis cDNA library. A nucleotide sequence of 2 173 bp long was determined to contain an open reading frame of 1 032 nucleotides (344 amino acids). In view of the homology to members of the galactosyltransferase gene family and especially the closest relationship to Gallus gallus GalT type I (CK I), the predicted product of the novel cDNA was designated as human β-1,4-galactosyltransferase homolog I (HumGT-H1). Its mRNA is present in different degrees in 16 tissues examined. Southern analysis of human genomic DNA revealed its locus on chromosome 3.

Original language	English (US)
Pages (from-to)	337-345
Number of pages	9
Journal	Science in China, Series C: Life Sciences
Volume	42
Issue number	4
DOIs	https://doi.org/10.1007/bf02882052
State	Published - Aug 1999

Keywords

Homolog
cDNA
β-1,4-galactosyltransferase

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology
General Environmental Science
General Agricultural and Biological Sciences

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10.1007/bf02882052

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title = "Cloning and characterization of a novel member of human β-1,4-galactosyltransferase gene family",

abstract = "By using the EST strategy for identifying novel members belonging to homologous gene families, a novel full-length cDNA encoding a protein significantly homologous to UDP-Gal: N-acetylglucosamine β-1,4-galactosyltransferase (GalT) was isolated from a human testis cDNA library. A nucleotide sequence of 2 173 bp long was determined to contain an open reading frame of 1 032 nucleotides (344 amino acids). In view of the homology to members of the galactosyltransferase gene family and especially the closest relationship to Gallus gallus GalT type I (CK I), the predicted product of the novel cDNA was designated as human β-1,4-galactosyltransferase homolog I (HumGT-H1). Its mRNA is present in different degrees in 16 tissues examined. Southern analysis of human genomic DNA revealed its locus on chromosome 3.",

keywords = "Homolog, cDNA, β-1,4-galactosyltransferase",

author = "Yuxin Fan and Long Yu and Qi Zhang and Ying Jiang and Fangyan Dai and Chiyuan Chen and Qiang Tu and Anding Bi and Yuefang Xu and Shouyuan Zhao",

year = "1999",

month = aug,

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AU - Fan, Yuxin

AU - Yu, Long

AU - Zhang, Qi

AU - Jiang, Ying

AU - Dai, Fangyan

AU - Chen, Chiyuan

AU - Tu, Qiang

AU - Bi, Anding

AU - Xu, Yuefang

AU - Zhao, Shouyuan

PY - 1999/8

Y1 - 1999/8

N2 - By using the EST strategy for identifying novel members belonging to homologous gene families, a novel full-length cDNA encoding a protein significantly homologous to UDP-Gal: N-acetylglucosamine β-1,4-galactosyltransferase (GalT) was isolated from a human testis cDNA library. A nucleotide sequence of 2 173 bp long was determined to contain an open reading frame of 1 032 nucleotides (344 amino acids). In view of the homology to members of the galactosyltransferase gene family and especially the closest relationship to Gallus gallus GalT type I (CK I), the predicted product of the novel cDNA was designated as human β-1,4-galactosyltransferase homolog I (HumGT-H1). Its mRNA is present in different degrees in 16 tissues examined. Southern analysis of human genomic DNA revealed its locus on chromosome 3.

AB - By using the EST strategy for identifying novel members belonging to homologous gene families, a novel full-length cDNA encoding a protein significantly homologous to UDP-Gal: N-acetylglucosamine β-1,4-galactosyltransferase (GalT) was isolated from a human testis cDNA library. A nucleotide sequence of 2 173 bp long was determined to contain an open reading frame of 1 032 nucleotides (344 amino acids). In view of the homology to members of the galactosyltransferase gene family and especially the closest relationship to Gallus gallus GalT type I (CK I), the predicted product of the novel cDNA was designated as human β-1,4-galactosyltransferase homolog I (HumGT-H1). Its mRNA is present in different degrees in 16 tissues examined. Southern analysis of human genomic DNA revealed its locus on chromosome 3.

KW - Homolog

KW - cDNA

KW - β-1,4-galactosyltransferase

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Cloning and characterization of a novel member of human β-1,4-galactosyltransferase gene family

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

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