Cloning and comparative sequence analysis of the gene encoding canine intercellular adhesion molecule-1 (ICAM-1)

Anthony M. Manning, Huifang Lu, Gilbert L. Kukielka, Mary G. Oliver, Theresa Ty, Carol A. Toman, Roger F. Drong, Jerry L. Slightom, Christie M. Ballantyne, Mark L. Entman, C. Wayne Smith, Donald C. Anderson

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Canine intercellular adhesion molecule-1 (ICAM-1) plays a primary role in the adherence of canine neutrophils to endothelial cells and in the cytotoxicity of canine neutrophils for adult cardiac myocytes. We have cloned the canine ICAM-1 gene and have analyzed the conservation of ICAM-1 amino acid (aa) sequences in man, chimpanzee, mouse, rat and dog. Canine ICAM-1 displays 61% identity with human ICAM-1. Cys residues critical to the immunoglobulin (Ig) fold structure and four sites of N-linked glycosylation are absolutely conserved in ICAM-1 from all species. Residues in the cytoplasmic tail associated with cytoskeletal α-actinin binding are highly conserved, supporting the hypothesis that intracellular attachment is indeed important for ICAM-1 function. Residues critical for human ICAM-1 binding to the β2-integrin leukocyte-function-associated antigen 1 (LFA-1) are highly conserved between all species, whereas those residues demonstrated to play an important role in interaction of human ICAM-1 with macrophage activation complex 1 (Mac-1) are not highly conserved. Residues critical for ICAM-1 binding to rhinovirus and malaria-infected red blood cells (IRBC) are not highly conserved.

Original languageEnglish (US)
Pages (from-to)291-295
Number of pages5
JournalGene
Volume156
Issue number2
DOIs
StatePublished - Apr 24 1995

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Intercellular Adhesion Molecule-1
Sequence Analysis
Canidae
Organism Cloning
Genes
Neutrophils
Actinin
Rhinovirus
Lymphocyte Function-Associated Antigen-1
Pan troglodytes
Macrophage Activation
Glycosylation
Cardiac Myocytes
Integrins
Malaria
Tail
Immunoglobulins
Amino Acid Sequence
Endothelial Cells
Erythrocytes

Keywords

  • Neutrophil
  • dog
  • immunoglobulin supergene family
  • inflammation

ASJC Scopus subject areas

  • Genetics

Cite this

Manning, A. M., Lu, H., Kukielka, G. L., Oliver, M. G., Ty, T., Toman, C. A., ... Anderson, D. C. (1995). Cloning and comparative sequence analysis of the gene encoding canine intercellular adhesion molecule-1 (ICAM-1). Gene, 156(2), 291-295. https://doi.org/10.1016/0378-1119(95)00045-8

Cloning and comparative sequence analysis of the gene encoding canine intercellular adhesion molecule-1 (ICAM-1). / Manning, Anthony M.; Lu, Huifang; Kukielka, Gilbert L.; Oliver, Mary G.; Ty, Theresa; Toman, Carol A.; Drong, Roger F.; Slightom, Jerry L.; Ballantyne, Christie M.; Entman, Mark L.; Smith, C. Wayne; Anderson, Donald C.

In: Gene, Vol. 156, No. 2, 24.04.1995, p. 291-295.

Research output: Contribution to journalArticle

Manning, AM, Lu, H, Kukielka, GL, Oliver, MG, Ty, T, Toman, CA, Drong, RF, Slightom, JL, Ballantyne, CM, Entman, ML, Smith, CW & Anderson, DC 1995, 'Cloning and comparative sequence analysis of the gene encoding canine intercellular adhesion molecule-1 (ICAM-1)', Gene, vol. 156, no. 2, pp. 291-295. https://doi.org/10.1016/0378-1119(95)00045-8
Manning, Anthony M. ; Lu, Huifang ; Kukielka, Gilbert L. ; Oliver, Mary G. ; Ty, Theresa ; Toman, Carol A. ; Drong, Roger F. ; Slightom, Jerry L. ; Ballantyne, Christie M. ; Entman, Mark L. ; Smith, C. Wayne ; Anderson, Donald C. / Cloning and comparative sequence analysis of the gene encoding canine intercellular adhesion molecule-1 (ICAM-1). In: Gene. 1995 ; Vol. 156, No. 2. pp. 291-295.
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