TY - JOUR
T1 - [Cloning, sequencing and identification of replication origin of Rhodococcus linear plasmid pNSL1].
AU - Zhu, Yingmin
AU - Xu, Mingxuan
AU - Shen, Meijuan
AU - Chen, Zhenhua
AU - Qin, Zhongjun
PY - 2010/8/4
Y1 - 2010/8/4
N2 - Two linear plasmids, pNSL1 and pNSL1, were detected from Rhodocuccus sp. NS1. OBJECTIVE: Cloning, sequencing and identification of replication origin of the Rhodococcus linear plasmid pNSL1. METHODS: Large amount of linear plasmid DNA was recovered from pulsed-field gels for shotgun-cloning and sequencing, and identification of its replication locus. RESULTS: The complete nucleotide sequence of pNSL1 consisted of 117252 bp, including the conserved 1282-bp telomere sequences among Rhodococcus linear plasmids. pNSL1 encoded 103 open reading frames, including functions of replication, maintenance and transfer etc. A locus, pNSL1. 038 and upstream 767-bp non-coding sequence, was identified for autonomous replication by cloning in an E. coli vector and introduced by electroporation into Nocardia coralline 4. 1037. CONCLUSION: Cloning and sequencing of Rhodococcus linear plasmid pNSL1, and identification of its replication origin.
AB - Two linear plasmids, pNSL1 and pNSL1, were detected from Rhodocuccus sp. NS1. OBJECTIVE: Cloning, sequencing and identification of replication origin of the Rhodococcus linear plasmid pNSL1. METHODS: Large amount of linear plasmid DNA was recovered from pulsed-field gels for shotgun-cloning and sequencing, and identification of its replication locus. RESULTS: The complete nucleotide sequence of pNSL1 consisted of 117252 bp, including the conserved 1282-bp telomere sequences among Rhodococcus linear plasmids. pNSL1 encoded 103 open reading frames, including functions of replication, maintenance and transfer etc. A locus, pNSL1. 038 and upstream 767-bp non-coding sequence, was identified for autonomous replication by cloning in an E. coli vector and introduced by electroporation into Nocardia coralline 4. 1037. CONCLUSION: Cloning and sequencing of Rhodococcus linear plasmid pNSL1, and identification of its replication origin.
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M3 - Article
C2 - 20931880
AN - SCOPUS:78049389113
SN - 0001-6209
VL - 50
SP - 1098
EP - 1103
JO - Wei sheng wu xue bao = Acta microbiologica Sinica
JF - Wei sheng wu xue bao = Acta microbiologica Sinica
IS - 8
ER -