TY - GEN
T1 - Combined photoacoustic and molecular fluorescence imaging in vivo
AU - Wang, Lihong V.
AU - Xie, Xueyi
AU - Oh, Jung Taek
AU - Li, Meng N.
AU - Ku, Geng
AU - Ke, Shi
AU - Similache, Sergiu
AU - Li, Chun
AU - Stoica, George
PY - 2005/1/1
Y1 - 2005/1/1
N2 - Because of the overwhelming scattering of light in biological tissues, the spatial resolution and imaging depth of conventional fluorescent imaging is unsatisfactory. Therefore, we present a dual modality imaging technique by combining fluorescence imaging with high-resolution noninvasive photoacoustic tomography (PAT) for the study of an animal tumor model. PAT provides high-resolution structural images of tumor angiogenesis, and fluorescence imaging offers high sensitivity to molecular probes for tumor detection. Coregistration of the PAT and fluorescence images was performed on nude mice with M21 human melanoma cell lines with αvβ3 integrin expression. An integrin αvβ3-targeted peptide-ICG conjugated NIR fluorescent contrast agent was used as the molecular probe for tumor detection. PAT was employed to noninvasively image the brain structure and the angiogenesis associated with tumors in mice. The coregistration between the PAT and fluorescence images was used to visualize tumor location, angiogenesis, and brain structure simultaneously.
AB - Because of the overwhelming scattering of light in biological tissues, the spatial resolution and imaging depth of conventional fluorescent imaging is unsatisfactory. Therefore, we present a dual modality imaging technique by combining fluorescence imaging with high-resolution noninvasive photoacoustic tomography (PAT) for the study of an animal tumor model. PAT provides high-resolution structural images of tumor angiogenesis, and fluorescence imaging offers high sensitivity to molecular probes for tumor detection. Coregistration of the PAT and fluorescence images was performed on nude mice with M21 human melanoma cell lines with αvβ3 integrin expression. An integrin αvβ3-targeted peptide-ICG conjugated NIR fluorescent contrast agent was used as the molecular probe for tumor detection. PAT was employed to noninvasively image the brain structure and the angiogenesis associated with tumors in mice. The coregistration between the PAT and fluorescence images was used to visualize tumor location, angiogenesis, and brain structure simultaneously.
UR - http://www.scopus.com/inward/record.url?scp=33846914919&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33846914919&partnerID=8YFLogxK
U2 - 10.1109/iembs.2005.1616374
DO - 10.1109/iembs.2005.1616374
M3 - Conference contribution
C2 - 17282143
AN - SCOPUS:33846914919
SN - 0780387406
SN - 9780780387409
T3 - Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
SP - 190
EP - 192
BT - Proceedings of the 2005 27th Annual International Conference of the Engineering in Medicine and Biology Society, IEEE-EMBS 2005
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 2005 27th Annual International Conference of the Engineering in Medicine and Biology Society, IEEE-EMBS 2005
Y2 - 1 September 2005 through 4 September 2005
ER -