Community-developed checklists for publishing images and image analyses

Christopher Schmied; Michael S. Nelson; Sergiy Avilov; Gert Jan Bakker; Cristina Bertocchi; Johanna Bischof; Ulrike Boehm; Jan Brocher; Mariana T. Carvalho; Catalin Chiritescu; Jana Christopher; Beth A. Cimini; Eduardo Conde-Sousa; Michael Ebner; Rupert Ecker; Kevin Eliceiri; Julia Fernandez-Rodriguez; Nathalie Gaudreault; Laurent Gelman; David Grunwald; Tingting Gu; Nadia Halidi; Mathias Hammer; Matthew Hartley; Marie Held; Florian Jug; Varun Kapoor; Ayse Aslihan Koksoy; Judith Lacoste; Sylvia Le Dévédec; Sylvie Le Guyader; Penghuan Liu; Gabriel G. Martins; Aastha Mathur; Kota Miura; Paula Montero Llopis; Roland Nitschke; Alison North; Adam C. Parslow; Alex Payne-Dwyer; Laure Plantard; Rizwan Ali; Britta Schroth-Diez; Lucas Schütz; Ryan T. Scott; Arne Seitz; Olaf Selchow; Ved P. Sharma; Martin Spitaler; Sathya Srinivasan; Caterina Strambio-De-Castillia; Douglas Taatjes; Christian Tischer; Helena Klara Jambor

doi:10.1038/s41592-023-01987-9

Community-developed checklists for publishing images and image analyses

Christopher Schmied, Michael S. Nelson, Sergiy Avilov, Gert Jan Bakker, Cristina Bertocchi, Johanna Bischof, Ulrike Boehm, Jan Brocher, Mariana T. Carvalho, Catalin Chiritescu, Jana Christopher, Beth A. Cimini, Eduardo Conde-Sousa, Michael Ebner, Rupert Ecker, Kevin Eliceiri, Julia Fernandez-Rodriguez, Nathalie Gaudreault, Laurent Gelman, David GrunwaldTingting Gu, Nadia Halidi, Mathias Hammer, Matthew Hartley, Marie Held, Florian Jug, Varun Kapoor, Ayse Aslihan Koksoy, Judith Lacoste, Sylvia Le Dévédec, Sylvie Le Guyader, Penghuan Liu, Gabriel G. Martins, Aastha Mathur, Kota Miura, Paula Montero Llopis, Roland Nitschke, Alison North, Adam C. Parslow, Alex Payne-Dwyer, Laure Plantard, Rizwan Ali, Britta Schroth-Diez, Lucas Schütz, Ryan T. Scott, Arne Seitz, Olaf Selchow, Ved P. Sharma, Martin Spitaler, Sathya Srinivasan, Caterina Strambio-De-Castillia, Douglas Taatjes, Christian Tischer, Helena Klara Jambor

Hematopathology

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Images document scientific discoveries and are prevalent in modern biomedical research. Microscopy imaging in particular is currently undergoing rapid technological advancements. However, for scientists wishing to publish obtained images and image-analysis results, there are currently no unified guidelines for best practices. Consequently, microscopy images and image data in publications may be unclear or difficult to interpret. Here, we present community-developed checklists for preparing light microscopy images and describing image analyses for publications. These checklists offer authors, readers and publishers key recommendations for image formatting and annotation, color selection, data availability and reporting image-analysis workflows. The goal of our guidelines is to increase the clarity and reproducibility of image figures and thereby to heighten the quality and explanatory power of microscopy data.

Original language	English (US)
Pages (from-to)	170-181
Number of pages	12
Journal	Nature Methods
Volume	21
Issue number	2
DOIs	https://doi.org/10.1038/s41592-023-01987-9
State	Published - Feb 2024

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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10.1038/s41592-023-01987-9

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Schmied, C., Nelson, M. S., Avilov, S., Bakker, G. J., Bertocchi, C., Bischof, J., Boehm, U., Brocher, J., Carvalho, M. T., Chiritescu, C., Christopher, J., Cimini, B. A., Conde-Sousa, E., Ebner, M., Ecker, R., Eliceiri, K., Fernandez-Rodriguez, J., Gaudreault, N., Gelman, L., ... Jambor, H. K. (2024). Community-developed checklists for publishing images and image analyses. Nature Methods, 21(2), 170-181. https://doi.org/10.1038/s41592-023-01987-9

Schmied, C, Nelson, MS, Avilov, S, Bakker, GJ, Bertocchi, C, Bischof, J, Boehm, U, Brocher, J, Carvalho, MT, Chiritescu, C, Christopher, J, Cimini, BA, Conde-Sousa, E, Ebner, M, Ecker, R, Eliceiri, K, Fernandez-Rodriguez, J, Gaudreault, N, Gelman, L, Grunwald, D, Gu, T, Halidi, N, Hammer, M, Hartley, M, Held, M, Jug, F, Kapoor, V, Koksoy, AA, Lacoste, J, Le Dévédec, S, Le Guyader, S, Liu, P, Martins, GG, Mathur, A, Miura, K, Montero Llopis, P, Nitschke, R, North, A, Parslow, AC, Payne-Dwyer, A, Plantard, L, Ali, R, Schroth-Diez, B, Schütz, L, Scott, RT, Seitz, A, Selchow, O, Sharma, VP, Spitaler, M, Srinivasan, S, Strambio-De-Castillia, C, Taatjes, D, Tischer, C & Jambor, HK 2024, 'Community-developed checklists for publishing images and image analyses', Nature Methods, vol. 21, no. 2, pp. 170-181. https://doi.org/10.1038/s41592-023-01987-9

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title = "Community-developed checklists for publishing images and image analyses",

abstract = "Images document scientific discoveries and are prevalent in modern biomedical research. Microscopy imaging in particular is currently undergoing rapid technological advancements. However, for scientists wishing to publish obtained images and image-analysis results, there are currently no unified guidelines for best practices. Consequently, microscopy images and image data in publications may be unclear or difficult to interpret. Here, we present community-developed checklists for preparing light microscopy images and describing image analyses for publications. These checklists offer authors, readers and publishers key recommendations for image formatting and annotation, color selection, data availability and reporting image-analysis workflows. The goal of our guidelines is to increase the clarity and reproducibility of image figures and thereby to heighten the quality and explanatory power of microscopy data.",

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AU - Boehm, Ulrike

AU - Brocher, Jan

AU - Carvalho, Mariana T.

AU - Chiritescu, Catalin

AU - Christopher, Jana

AU - Cimini, Beth A.

AU - Conde-Sousa, Eduardo

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AU - Eliceiri, Kevin

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AU - Miura, Kota

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AU - North, Alison

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AU - Seitz, Arne

AU - Selchow, Olaf

AU - Sharma, Ved P.

AU - Spitaler, Martin

AU - Srinivasan, Sathya

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Community-developed checklists for publishing images and image analyses

Abstract

ASJC Scopus subject areas

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