Complex interaction of deferasirox and pythium insidiosum: Iron-dependent attenuation of growth in vitro and immunotherapy-like enhancement of immune responses in vivo

Régis A. Zanette; Paula E.R. Bitencourt; Dimitrios P. Kontoyiannis; Rafael A. Fighera; Mariana M. Flores; Glaucia D. Kommers; Priscila S. Silva; Aline Ludwig; Maria B. Moretto; Sydney H. Alves; Janio M. Santurio

doi:10.1371/journal.pone.0118932

Complex interaction of deferasirox and pythium insidiosum: Iron-dependent attenuation of growth in vitro and immunotherapy-like enhancement of immune responses in vivo

Régis A. Zanette, Paula E.R. Bitencourt, Dimitrios P. Kontoyiannis, Rafael A. Fighera, Mariana M. Flores, Glaucia D. Kommers, Priscila S. Silva, Aline Ludwig, Maria B. Moretto, Sydney H. Alves, Janio M. Santurio

Infectious Diseases, Infection Control, and Employee Health

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Abstract

Pythium insidiosum iron acquisition mechanisms are unknown. We previously showed that the iron chelator deferasirox had weak activity in vitro and in rabbits with experimental pythiosis. Here we show that deferasirox causes damage to P. insidiosum hyphae in vitro, but that activity is diminished in the presence of exogenous iron. The tissue activity of the proinflammatory enzyme adenosine deaminase and the histological pattern observed in pythiosis lesions of rabbits treated with deferasirox were similar to the ones in animals treated with immunotherapy.

Original language	English (US)
Article number	e0118932
Journal	PloS one
Volume	10
Issue number	3
DOIs	https://doi.org/10.1371/journal.pone.0118932
State	Published - Mar 4 2015

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology
General Agricultural and Biological Sciences
General

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Zanette, R. A., Bitencourt, P. E. R., Kontoyiannis, D. P., Fighera, R. A., Flores, M. M., Kommers, G. D., Silva, P. S., Ludwig, A., Moretto, M. B., Alves, S. H., & Santurio, J. M. (2015). Complex interaction of deferasirox and pythium insidiosum: Iron-dependent attenuation of growth in vitro and immunotherapy-like enhancement of immune responses in vivo. PloS one, 10(3), Article e0118932. https://doi.org/10.1371/journal.pone.0118932

Zanette, RA, Bitencourt, PER, Kontoyiannis, DP, Fighera, RA, Flores, MM, Kommers, GD, Silva, PS, Ludwig, A, Moretto, MB, Alves, SH & Santurio, JM 2015, 'Complex interaction of deferasirox and pythium insidiosum: Iron-dependent attenuation of growth in vitro and immunotherapy-like enhancement of immune responses in vivo', PloS one, vol. 10, no. 3, e0118932. https://doi.org/10.1371/journal.pone.0118932

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abstract = "Pythium insidiosum iron acquisition mechanisms are unknown. We previously showed that the iron chelator deferasirox had weak activity in vitro and in rabbits with experimental pythiosis. Here we show that deferasirox causes damage to P. insidiosum hyphae in vitro, but that activity is diminished in the presence of exogenous iron. The tissue activity of the proinflammatory enzyme adenosine deaminase and the histological pattern observed in pythiosis lesions of rabbits treated with deferasirox were similar to the ones in animals treated with immunotherapy.",

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AU - Kommers, Glaucia D.

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Complex interaction of deferasirox and pythium insidiosum: Iron-dependent attenuation of growth in vitro and immunotherapy-like enhancement of immune responses in vivo

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