Concurrent flow cytometric analysis of DNA and RNA.

Adel K. El-Naggar

doi:10.1385/1-59259-773-4:371

Concurrent flow cytometric analysis of DNA and RNA.

Adel K. El-Naggar

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Concurrent analysis of DNA and RNA by flow cytometry provides information on the DNA content and the transcriptional status of cells. This can be accomplished using metachromatic fluorochromes that bind to DNA by intercalation and to single-stranded RNA electrostatically. Because cell viability is a prerequisite for the analysis, freshly prepared cells must be used. Simultaneous DNA/RNA analysis can be used primarily in the classifications and biological assessment of hematoreticular malignancies including multiple myeloma.

Original language	English (US)
Pages (from-to)	371-384
Number of pages	14
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	263
DOIs	https://doi.org/10.1385/1-59259-773-4:371
State	Published - 2004
Externally published	Yes

ASJC Scopus subject areas

Molecular Biology
Genetics

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AB - Concurrent analysis of DNA and RNA by flow cytometry provides information on the DNA content and the transcriptional status of cells. This can be accomplished using metachromatic fluorochromes that bind to DNA by intercalation and to single-stranded RNA electrostatically. Because cell viability is a prerequisite for the analysis, freshly prepared cells must be used. Simultaneous DNA/RNA analysis can be used primarily in the classifications and biological assessment of hematoreticular malignancies including multiple myeloma.

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Concurrent flow cytometric analysis of DNA and RNA.

Abstract

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