TY - JOUR
T1 - Constitutive and Induced Expression of Growth Factors in Normal and Chronic Phase Chronic Myelogenous Leukemia Ph1 Bone Marrow Stroma
AU - Wetzler, Meir
AU - Kurzrock, Razelle
AU - Taylor, Kerry
AU - Spitzer, Gary
AU - Kantarjian, Hagop
AU - Baiocchi, Gabriella
AU - Ku, Stella
AU - Gutterman, Jordan U.
AU - Talpaz, Moshe
N1 - Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 1990/10/1
Y1 - 1990/10/1
N2 - Study of growth factor RNA levels in the stromal cells derived from the adherent layer of long-term bone marrow culture demonstrated constitutive expression of transforming growth factor βt (TGF-0i) and macrophage colony-stimulating factor. These cells did not express granulocyte colony-stimulating factor, granulocyte-monocyte colony-stimulating factor, interleukin (IL) lα, IL-1β, IL-3, and IL-6. However, granulocyte colony-stimulating factor expression could be induced by recombinant human IL-10; while IL-6 could be induced by both IL-lβ and tumor necrosis factor-a. No differences could be detected between adherent layers established from normal and benign phase Ph1 chronic myelogenous leukemia bone marrow. The uninduced expression of TGF-01, a potent hematopoietic cell growth inhibitor, suggests that stromal cells play an inherent role in regulating the proliferation of adjacent bone marrow hematopoietic progenitor cells. However, a defect in stromal TGF-01 production cannot account for the profoundly expanded myeloid compartment in chronic phase chronic myelogenous leukemia. In contrast to the constitutive expression of TGF-0( and macrophage colony-stimulating factor, hematopoietic growth factors are only expressed following a proper stimulation.
AB - Study of growth factor RNA levels in the stromal cells derived from the adherent layer of long-term bone marrow culture demonstrated constitutive expression of transforming growth factor βt (TGF-0i) and macrophage colony-stimulating factor. These cells did not express granulocyte colony-stimulating factor, granulocyte-monocyte colony-stimulating factor, interleukin (IL) lα, IL-1β, IL-3, and IL-6. However, granulocyte colony-stimulating factor expression could be induced by recombinant human IL-10; while IL-6 could be induced by both IL-lβ and tumor necrosis factor-a. No differences could be detected between adherent layers established from normal and benign phase Ph1 chronic myelogenous leukemia bone marrow. The uninduced expression of TGF-01, a potent hematopoietic cell growth inhibitor, suggests that stromal cells play an inherent role in regulating the proliferation of adjacent bone marrow hematopoietic progenitor cells. However, a defect in stromal TGF-01 production cannot account for the profoundly expanded myeloid compartment in chronic phase chronic myelogenous leukemia. In contrast to the constitutive expression of TGF-0( and macrophage colony-stimulating factor, hematopoietic growth factors are only expressed following a proper stimulation.
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M3 - Article
C2 - 2203522
AN - SCOPUS:0025147259
SN - 0008-5472
VL - 50
SP - 5801
EP - 5805
JO - Cancer Research
JF - Cancer Research
IS - 18
ER -