TY - JOUR
T1 - Constitutive and inducible nuclear factor-κB in immortalized normal human bronchial epithelial and non-small cell lung cancer cell lines
AU - Baby, Johnson
AU - Pickering, Brian F.
AU - Vashisht Gopal, Y. N.
AU - Van Dyke, Michael W.
PY - 2007/9/18
Y1 - 2007/9/18
N2 - Constitutive activation of the proinflammatory nuclear factor κB (NF-κB) transcription factor p65RelA/p50 has been implicated in many cancers, including leukemias, lymphomas, and several solid tumors, including lung cancer. In many cases, constitutive NF-κB activation can be recapitulated in cell lines isolated from these cancers. To test whether this is the case with non-small cell lung cancer (NSCLC) cell lines, we investigated the basal levels of NF-κB proteins, their subcellular distribution, their DNA-binding activities, and the expression of NF-κB-responsive genes in 10 NSCLC cell lines. The immortalized human bronchial epithelial cell line BEAS-2B served as a normal control. We found little evidence of substantial constitutive NF-κB activation in NSCLC cell lines, although most all of the normal and NSCLC cells possessed inducible NF-κB. Our findings provide a resource for the use of particular NSCLC cell lines for the investigation of constitutive and inducible NF-κB activity in vitro.
AB - Constitutive activation of the proinflammatory nuclear factor κB (NF-κB) transcription factor p65RelA/p50 has been implicated in many cancers, including leukemias, lymphomas, and several solid tumors, including lung cancer. In many cases, constitutive NF-κB activation can be recapitulated in cell lines isolated from these cancers. To test whether this is the case with non-small cell lung cancer (NSCLC) cell lines, we investigated the basal levels of NF-κB proteins, their subcellular distribution, their DNA-binding activities, and the expression of NF-κB-responsive genes in 10 NSCLC cell lines. The immortalized human bronchial epithelial cell line BEAS-2B served as a normal control. We found little evidence of substantial constitutive NF-κB activation in NSCLC cell lines, although most all of the normal and NSCLC cells possessed inducible NF-κB. Our findings provide a resource for the use of particular NSCLC cell lines for the investigation of constitutive and inducible NF-κB activity in vitro.
KW - EMSA
KW - Human bronchial epithelial cells
KW - Non-small cell lung carcinoma
KW - Nuclear factor-κB
KW - TNF-α
UR - http://www.scopus.com/inward/record.url?scp=34547686047&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34547686047&partnerID=8YFLogxK
U2 - 10.1016/j.canlet.2007.03.024
DO - 10.1016/j.canlet.2007.03.024
M3 - Article
C2 - 17493745
AN - SCOPUS:34547686047
SN - 0304-3835
VL - 255
SP - 85
EP - 94
JO - Cancer Letters
JF - Cancer Letters
IS - 1
ER -