Abstract
Aim: Previously, it was shown that treatment of tumor-bearing mice with an RNA replicase-based plasmid that produces dsRNA when transfected into tumor cells significantly inhibited the tumor growth. In the present study, the feasibility of further improving the anti-tumor activity of the RNA replicase-based plasmid by targeting it into tumors cells was evaluated. Material & methods: An EGF-conjugated, polyethylene glycosylated cationic liposome was developed to deliver the RNA replicase-based plasmid, pSIN-β, into EGF receptor-overexpressing human breast cancer cells (MDA-MB-468) in vitro and in vivo. Results: Delivery of pSIN-β using the EGF receptor-targeted liposome more effectively controlled the growth of MDA-MB-468 tumors (and human epidermoid carcinoma A431 tumors) in mice than using untargeted liposome. The pSIN-β carried by the EGF receptor-targeted liposome caused the complete regression of MDA-MB-468 tumors in mice, probably due to the enhancement of its proapoptotic, antiproliferative and antiangiogenic activities. Discussion: Tumor-targeted RNA replicase-based plasmids hold a strong potential in tumor therapy.
Original language | English (US) |
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Pages (from-to) | 475-491 |
Number of pages | 17 |
Journal | Nanomedicine |
Volume | 7 |
Issue number | 4 |
DOIs | |
State | Published - Apr 2012 |
Externally published | Yes |
Keywords
- apoptosis
- breast tumor
- dsRNA therapy
- epidermoid carcinoma
- liposome
- plasmid uptake
ASJC Scopus subject areas
- Bioengineering
- Medicine (miscellaneous)
- Biomedical Engineering
- General Materials Science