TY - JOUR
T1 - Correlation of growth capacity of human tumor cells in hard agarose with their in vivo proliferative capacity at specific metastatic sites
AU - Li, Limin
AU - Price, Janet E.
AU - Fan, Dominic
AU - Zhang, Ruo dan
AU - Bucana, Corazon D.
AU - Fidler, Isaiah J.
N1 - Funding Information:
Received May 19, 1989; accepted June 26, 1989. Supported in part by funds from Triton Biosciences, Inc., and by Public Health Service grants CA-42107 (National Cancer Institute) and RR-5511-23 (Division of Research Resources), National Institutes of Health, Department of Health and Human Services. Department of Cell Biology, The University of Texas M.D. Anderson CancerCenter, Houston, TX. We thank Christopher Seid for technical assistance and Lola Lopez for help in the preparation of this manuscript. *Correspondence to: Dr. L J. Fidler, Department of Cell Biology, Box 173, The University of Texas M.D. Anderson Cancer Center, 1515 Holcombe Blvd., Houston, TX 77030.
PY - 1989/9/20
Y1 - 1989/9/20
N2 - The purpose of this study was to determine whether the degree of anchorage-independent growth of human tumor cells in increasing concentrations of agarose correlated with the capacity of the cells to produce experimental metastases in nude mice. Human melanoma, breast carcinoma, and colon carcinoma cells from parental lines and variants selected in vivo for metastasis and in vitro cloned lines were plated into medium containing 0.3%, 0.6%, 0.9%, or 1.2% of agarose. These cells were also injected into nude mice: intravenously for melanoma, into the mammary fat pad for breast carcinoma, and into the spleen for colon carcinoma. Production of tumor cell colonies in dense agarose (>0.6%) correlated with production of experimental metastases in the lung (melanoma, breast carcinoma) or liver (colon carcinoma). We conclude that the degree of anchorage-independent growth of tumor cells can predict their biological behavior and metastatic potential in vivo. Thus, this technique may be useful for the isolation of metastátic cells from heterogeneous human neoplasms. [J Nati Cancer Inst 81:1406- 1412, 1989].
AB - The purpose of this study was to determine whether the degree of anchorage-independent growth of human tumor cells in increasing concentrations of agarose correlated with the capacity of the cells to produce experimental metastases in nude mice. Human melanoma, breast carcinoma, and colon carcinoma cells from parental lines and variants selected in vivo for metastasis and in vitro cloned lines were plated into medium containing 0.3%, 0.6%, 0.9%, or 1.2% of agarose. These cells were also injected into nude mice: intravenously for melanoma, into the mammary fat pad for breast carcinoma, and into the spleen for colon carcinoma. Production of tumor cell colonies in dense agarose (>0.6%) correlated with production of experimental metastases in the lung (melanoma, breast carcinoma) or liver (colon carcinoma). We conclude that the degree of anchorage-independent growth of tumor cells can predict their biological behavior and metastatic potential in vivo. Thus, this technique may be useful for the isolation of metastátic cells from heterogeneous human neoplasms. [J Nati Cancer Inst 81:1406- 1412, 1989].
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U2 - 10.1093/jnci/81.18.1406
DO - 10.1093/jnci/81.18.1406
M3 - Article
C2 - 2778827
AN - SCOPUS:0024443170
SN - 0027-8874
VL - 81
SP - 1406
EP - 1412
JO - Journal of the National Cancer Institute
JF - Journal of the National Cancer Institute
IS - 18
ER -