TY - JOUR
T1 - Counting Murine Corpora Lutea to Determine the Number of Oocytes Ovulated
AU - Papaioannou, Virginia E.
AU - Behringer, Richard R.
N1 - Publisher Copyright:
© 2023 Cold Spring Harbor Laboratory Press.
PY - 2023/12
Y1 - 2023/12
N2 - One can determine if and how many oocytes have been ovulated in a female mouse by counting the number of corpora lutea (CL) on the ovaries during the process of preimplantation embryo collection. A simple method of harvesting the ovaries and observing with a dissecting microscope and top lighting is provided along with a description of how to recognize CL. As the embryos rarely, if ever, cross the uterotubal junction, this provides a measure of the maximum number of embryos expected to be recovered from each oviduct or uterine horn, a number that can be valuable in analyzing early lethal mutations.
AB - One can determine if and how many oocytes have been ovulated in a female mouse by counting the number of corpora lutea (CL) on the ovaries during the process of preimplantation embryo collection. A simple method of harvesting the ovaries and observing with a dissecting microscope and top lighting is provided along with a description of how to recognize CL. As the embryos rarely, if ever, cross the uterotubal junction, this provides a measure of the maximum number of embryos expected to be recovered from each oviduct or uterine horn, a number that can be valuable in analyzing early lethal mutations.
UR - http://www.scopus.com/inward/record.url?scp=85181987663&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85181987663&partnerID=8YFLogxK
U2 - 10.1101/pdb.prot107986
DO - 10.1101/pdb.prot107986
M3 - Article
C2 - 37932081
AN - SCOPUS:85181987663
SN - 1940-3402
VL - 2023
SP - 984
EP - 986
JO - Cold Spring Harbor protocols
JF - Cold Spring Harbor protocols
IS - 12
ER -