Abstract
The coxsackievirus and adenovirus receptor (CAR) is a transmembrane protein that is known to be a site of viral attachment and entry, but its physiologic functions are undefined. CAR expression is maximal in neonates and wanes rapidly after birth in organs such as heart, muscle, and brain, suggesting that CAR plays a role in the development of these tissues. Here, we show that CAR deficiency resulted in an embryonic lethal condition associated with cardiac defects. Specifically, commencing ∼10.5 days postconception (dpc), CAR -/- cardiomyocytes exhibited regional apoptosis evidenced by both histopathologic features of cell death and positive staining for the apoptotic marker cleaved caspase 3. CAR-/- fetuses invariably suffered from degeneration of the myocardial wall and thoracic hemorrhaging, leading to death by 11.5 dpc. These findings are consistent with the view that CAR provides positive survival signals to cardiomyocytes that are essential for normal heart development.
Original language | English (US) |
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Pages (from-to) | 77-85 |
Number of pages | 9 |
Journal | Genesis (United States) |
Volume | 42 |
Issue number | 2 |
DOIs | |
State | Published - Jun 2005 |
Externally published | Yes |
Keywords
- CAR
- Cardiomyocyte development
- Coxsackievirus and Adenovirus Receptor
- Embryonic lethal
- Heart organogenesis
- Knockout mouse
- Targeted deletion
ASJC Scopus subject areas
- Genetics
- Endocrinology
- Cell Biology