Cross-level sampling for bandwidth effective time-domain fluorescence lifetime imaging implementation

Elvis Duran, Sebastian Hoyos, Javier Jo

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The implementation of time domain fluorescence lifetime imaging (TD-FLIM) requires high cost bandwidth electronics and large storage capacity. A cross-level sampling technique for TD-FLIM is proposed. A simulation of this sampling technique was implemented using synthetic FLIM data. FLIM images were synthetically generated at different noise levels using a wide range of lifetimes. Each of the images was resampled using a cross-level approach and the lifetime maps were computed. Simulation results displayed strong correlation between the lifetime maps of the original and resampled images, suggesting that this sampling method could be adopted to reduce bandwidth and data transfer/ storage requirements.

Original languageEnglish (US)
Title of host publicationThree-Dimensional and Multidimensional Microscopy
Subtitle of host publicationImage Acquisition and Processing XXV
EditorsTony Wilson, Carol J. Cogswell, Thomas G. Brown
PublisherSPIE
ISBN (Electronic)9781510614833
DOIs
StatePublished - 2018
EventThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV 2018 - San Francisco, United States
Duration: Jan 29 2018Jan 31 2018

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume10499
ISSN (Print)1605-7422

Conference

ConferenceThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV 2018
Country/TerritoryUnited States
CitySan Francisco
Period1/29/181/31/18

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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