Abstract
High-pressure liquid chromatography has been used to separate the derivatives of guanosine which are formed when this nucleoside is reacted with busulfan. Derivatives which have been identified by a combination of ultraviolet and mass spectrometry include: 7-(δ-hydroxybutyl)guanosine, 1,4-di(7-guanosyl)butane, and 1-(7-guanyl)-4-(7-guanosinyl)butane. The latter two derivatives can be converted to 1,4-di(7-guanyl)butane by mild acid hydrolysis. Using 1,4-di(7-guanyl)butane as a marker for high-pressure liquid chromatography, we have identified this compound as a product of the reaction between DNA and busulfan. These findings verify the previously unconfirmed report that busulfan is a crosslinking agent for DNA and strengthen the hypothesis that differences in the biological properties of alkylating agents result from differences in their reactions with DNA.
Original language | English (US) |
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Pages (from-to) | 174-181 |
Number of pages | 8 |
Journal | BBA Section Nucleic Acids And Protein Synthesis |
Volume | 608 |
Issue number | 1 |
DOIs | |
State | Published - Jun 27 1980 |
Keywords
- Alkylating agent
- Antitumor compound
- Busulfan
- DNA crosslinking
ASJC Scopus subject areas
- General Medicine