Abstract
It was the purpose of this study to establish and evaluate a freezing and-thawing method for preservation of hemopoietic stem cells from the peripheral blood. Blood leukocytes collected by means of an IBM Blood-Cell-Separator were frozen in plastic bags using 10% DMSO and controlled cooling rates. Thawing was performed rapidly, and DMSO was diluted and removed prior to the in-vitro and in-vivo assays. The mean recovery of mononuclear cells collected from 82 leukaphereses was 86%. To assess the recovery of cryopreserved hemopoietic stem cells, the soft agar culture method adapted for the dog was used. There was no significant difference in the CFUc recovery per 1 × 106 mononuclear cells or in per leukapheresis after different Cryopreservation times (1-6 and 7-27 months). To evaluate the hemopoietic repopulation capability of cryopreserved blood stem cells, leukapheresis-derived leukocytes were transfused into 1200 R whole body x-irradiated dogs. The hemopoietic repopulation pattern at day 10 after transfusion of comparable numbers of fresh or frozen leukocytes was not significantly different, as measured in bone marrow smears and sections and by granulocyte concentration in the peripheral blood.
Original language | English (US) |
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Pages (from-to) | 195-202 |
Number of pages | 8 |
Journal | Blut |
Volume | 35 |
Issue number | 3 |
DOIs | |
State | Published - Sep 1977 |
Keywords
- Blood stem cells
- Cryopreservation
- DMSO
- Leukapheresis
- Marrow repopulating ability
ASJC Scopus subject areas
- Hematology