CTF determination of images of ice-embedded single particles using a graphics interface

Z. Hong Zhou; Steve Hardt; Bin Wang; Michael B. Sherman; Joanita Jakana; Wah Chiu

doi:10.1006/jsbi.1996.0033

CTF determination of images of ice-embedded single particles using a graphics interface

Z. Hong Zhou, Steve Hardt, Bin Wang, Michael B. Sherman, Joanita Jakana, Wah Chiu

Research output: Contribution to journal › Article › peer-review

87 Scopus citations

Abstract

We implement a graphical user interface in an X-window/UNIX environment to compute and display the incoherently averaged Fourier transforms of electron images of single particles embedded in ice and the simulated contrast transfer function with or without envelope functions, This interface provides an easy and efficient operation for the determination of defocus value and the evaluation of the extent of Fourier amplitude falloff, This computational procedure is crucial for prescreening image data and performing image correction of contrast transfer function in high-resolution three-dimensional reconstruction of single particles.

Original language	English (US)
Pages (from-to)	216-222
Number of pages	7
Journal	Journal of Structural Biology
Volume	116
Issue number	1
DOIs	https://doi.org/10.1006/jsbi.1996.0033
State	Published - Jan 1996
Externally published	Yes

ASJC Scopus subject areas

Structural Biology

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10.1006/jsbi.1996.0033

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title = "CTF determination of images of ice-embedded single particles using a graphics interface",

abstract = "We implement a graphical user interface in an X-window/UNIX environment to compute and display the incoherently averaged Fourier transforms of electron images of single particles embedded in ice and the simulated contrast transfer function with or without envelope functions, This interface provides an easy and efficient operation for the determination of defocus value and the evaluation of the extent of Fourier amplitude falloff, This computational procedure is crucial for prescreening image data and performing image correction of contrast transfer function in high-resolution three-dimensional reconstruction of single particles.",

author = "Zhou, {Z. Hong} and Steve Hardt and Bin Wang and Sherman, {Michael B.} and Joanita Jakana and Wah Chiu",

note = "Funding Information: This research has been supported by grants from National Institutes of Health (RR02250, GM08280, LM07093), National Science Foundation (BIR9413229), and the W. M. Keck Foundation. S.H. was a recipient of a Microscopy Society of America undergraduate scholarship. We thank Drs. Michael F. Schmid, A.",

year = "1996",

month = jan,

doi = "10.1006/jsbi.1996.0033",

language = "English (US)",

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AU - Zhou, Z. Hong

AU - Hardt, Steve

AU - Wang, Bin

AU - Sherman, Michael B.

AU - Jakana, Joanita

AU - Chiu, Wah

N1 - Funding Information: This research has been supported by grants from National Institutes of Health (RR02250, GM08280, LM07093), National Science Foundation (BIR9413229), and the W. M. Keck Foundation. S.H. was a recipient of a Microscopy Society of America undergraduate scholarship. We thank Drs. Michael F. Schmid, A.

PY - 1996/1

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AB - We implement a graphical user interface in an X-window/UNIX environment to compute and display the incoherently averaged Fourier transforms of electron images of single particles embedded in ice and the simulated contrast transfer function with or without envelope functions, This interface provides an easy and efficient operation for the determination of defocus value and the evaluation of the extent of Fourier amplitude falloff, This computational procedure is crucial for prescreening image data and performing image correction of contrast transfer function in high-resolution three-dimensional reconstruction of single particles.

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CTF determination of images of ice-embedded single particles using a graphics interface

Abstract

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