Abstract
A cascade of events is triggered upon the addition of growth factor to quiescent mammalian cells, which ultimately restarts proliferation by inducing the transition from G0/G1 to S-phase. We have studied cyclin D1, a putative G1 cyclin, in normal diploid human fibroblasts. Cyclin D1 accumulated and reached a maximum level before S-phase upon the addition of serum to quiescent cells. The protein was localized to the nucleus, and it disappeared from the nucleus as cells proceeded into S-phase. Mictoinjection of anti-cyclin D1 antibodies or antisense plasmid prevented cells from entering S-phase, and the kinetics of inhibition showed that cyclin D1 is required at a point in the cell cycle earlier than cyclin A. These results demonstrate that cyclin D1 is a critical target of proliferative signals in G1.
Original language | English (US) |
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Pages (from-to) | 812-821 |
Number of pages | 10 |
Journal | Genes and Development |
Volume | 7 |
Issue number | 5 |
DOIs | |
State | Published - 1993 |
Externally published | Yes |
Keywords
- Cell cycle
- Cell proliferation
- Cyclin D1
- Nuclear protein
ASJC Scopus subject areas
- Genetics
- Developmental Biology