Cyclosporin A-induced changes in nitric oxide synthase activity in the rat kidney: In vitro and in vivo assay

M. R. Rao; V. K. Kanji; V. V. Setty; C. Wang; A. K. Salahudeen; S. K. Das; H. H.P. Cohly

Cyclosporin A-induced changes in nitric oxide synthase activity in the rat kidney: In vitro and in vivo assay

M. R. Rao, V. K. Kanji, V. V. Setty, C. Wang, A. K. Salahudeen, S. K. Das, H. H.P. Cohly

General Internal Medicine

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Cyclosporin A (CsA) is a cyclic undecapeptide that has been used extensively as an immunosuppressive drug in transplantation medicine and is known to interact with L-arginine dependent pathways. We studied the in vitro and in vivo effects of CsA on nitric oxide synthase (NOS) activity in the rat kidney. CsA in concentrations of 44-2200 nM in vitro, and 12.5 or 25 mg/kg body weight per 4 weeks treated rat kidneys in vivo, significantly stimulated NOS activity. CsA may alter the Ca²⁺/Cam-dependent NOS activity by interacting with rat kidney Ca²⁺/calmodulin dependent events.

Original language	English (US)
Pages (from-to)	183-185
Number of pages	3
Journal	Indian Journal of Physiology and Pharmacology
Volume	40
Issue number	2
State	Published - 1996

Keywords

cyclosporin A
nephrotoxicity
nitric oxide synthase

ASJC Scopus subject areas

Physiology
Pharmacology
Physiology (medical)

Cite this

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title = "Cyclosporin A-induced changes in nitric oxide synthase activity in the rat kidney: In vitro and in vivo assay",

abstract = "Cyclosporin A (CsA) is a cyclic undecapeptide that has been used extensively as an immunosuppressive drug in transplantation medicine and is known to interact with L-arginine dependent pathways. We studied the in vitro and in vivo effects of CsA on nitric oxide synthase (NOS) activity in the rat kidney. CsA in concentrations of 44-2200 nM in vitro, and 12.5 or 25 mg/kg body weight per 4 weeks treated rat kidneys in vivo, significantly stimulated NOS activity. CsA may alter the Ca2+/Cam-dependent NOS activity by interacting with rat kidney Ca2+/calmodulin dependent events.",

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T1 - Cyclosporin A-induced changes in nitric oxide synthase activity in the rat kidney

T2 - In vitro and in vivo assay

AU - Rao, M. R.

AU - Kanji, V. K.

AU - Setty, V. V.

AU - Wang, C.

AU - Salahudeen, A. K.

AU - Das, S. K.

AU - Cohly, H. H.P.

PY - 1996

Y1 - 1996

N2 - Cyclosporin A (CsA) is a cyclic undecapeptide that has been used extensively as an immunosuppressive drug in transplantation medicine and is known to interact with L-arginine dependent pathways. We studied the in vitro and in vivo effects of CsA on nitric oxide synthase (NOS) activity in the rat kidney. CsA in concentrations of 44-2200 nM in vitro, and 12.5 or 25 mg/kg body weight per 4 weeks treated rat kidneys in vivo, significantly stimulated NOS activity. CsA may alter the Ca2+/Cam-dependent NOS activity by interacting with rat kidney Ca2+/calmodulin dependent events.

AB - Cyclosporin A (CsA) is a cyclic undecapeptide that has been used extensively as an immunosuppressive drug in transplantation medicine and is known to interact with L-arginine dependent pathways. We studied the in vitro and in vivo effects of CsA on nitric oxide synthase (NOS) activity in the rat kidney. CsA in concentrations of 44-2200 nM in vitro, and 12.5 or 25 mg/kg body weight per 4 weeks treated rat kidneys in vivo, significantly stimulated NOS activity. CsA may alter the Ca2+/Cam-dependent NOS activity by interacting with rat kidney Ca2+/calmodulin dependent events.

KW - cyclosporin A

KW - nephrotoxicity

KW - nitric oxide synthase

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Cyclosporin A-induced changes in nitric oxide synthase activity in the rat kidney: In vitro and in vivo assay

Abstract

Keywords

ASJC Scopus subject areas

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