Abstract
Cullins assemble the largest family of ubiquitin ligases by binding with ROC1 and various substrate receptors. CUL4 function is linked with many cellular processes, but its substrate-recruiting mechanism remains elusive. We identified a protein motif, the DWD box (DDB1-binding WD40 protein), and demonstrated the binding of 15 DWD proteins with DDB1-CUL4A. We provide evidence supporting the critical function of the DWD box and DDB1's role as the linker mediating DWD protein association with CUL4A. A database search predicts that about one-third of WD40 proteins, 90 in humans, contain the DWD box, suggesting a potentially large number of DWD-DDB1-CUL4-ROC1 E3 ligases.
Original language | English (US) |
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Pages (from-to) | 2949-2954 |
Number of pages | 6 |
Journal | Genes and Development |
Volume | 20 |
Issue number | 21 |
DOIs | |
State | Published - Nov 1 2006 |
Externally published | Yes |
Keywords
- CUL4
- Cullin
- DDB1
- DWD
- Ubiquitin
- WD40
ASJC Scopus subject areas
- Genetics
- Developmental Biology