Abstract
Altered genomic methylcytosine content has been described for a number of tumor types, including neuroblastoma. However, it remains to be determined for different tumor types whether specific loci or chromosomal regions are affected by a methylation change or whether the change is random. We have implemented a computer-based approach for the analysis of two-dimensional separations of human genomic restriction fragments. Through the use of methylation-sensitive restriction enzymes, methylation differences in genomic DNA between tumor and normal tissues can be detected. We report the cloning and sequencing of two fragments detectable in two-dimensional separations of genomic DNA of neuroblastomas. These fragments were found to be a part of repetitive units that exhibited demethylation in neuroblastoma relative to other tumor types. Our finding of a distinct pattern of methylation of repetitive units in neuroblastoma suggests that altered methylation at certain loci may contribute to the biology of this tumor.
Original language | English (US) |
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Pages (from-to) | 234-244 |
Number of pages | 11 |
Journal | Genes Chromosomes and Cancer |
Volume | 17 |
Issue number | 4 |
DOIs | |
State | Published - Dec 1996 |
Externally published | Yes |
ASJC Scopus subject areas
- Genetics
- Cancer Research