Depth resolved photothermal OCT detection of macrophages in tissue using nanorose

Amit S. Paranjape; Roman Kuranov; Stepan Baranov; Li Leo Ma; Joseph W. Villard; Tianyi Wang; Konstantin V. Sokolov; Marc D. Feldman; Keith P. Johnston; Thomas E. Minier

doi:10.1364/BOE.1.000002

Depth resolved photothermal OCT detection of macrophages in tissue using nanorose

Amit S. Paranjape, Roman Kuranov, Stepan Baranov, Li Leo Ma, Joseph W. Villard, Tianyi Wang, Konstantin V. Sokolov, Marc D. Feldman, Keith P. Johnston, Thomas E. Minier

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

Application of photothermal Optical Coherence Tomography (OCT) to detect macrophages in ex vivo rabbit arteries which have engulfed nanoclusters of gold coated iron oxide (nanorose) is reported. Nanorose engulfed by macrophages associated with atherosclerotic lesions in rabbit arteries absorb incident laser (800nm) energy and cause optical pathlength (OP) variation which is measured using photothermal OCT. OP variation in polydimethyl siloxane tissue phantoms containing varying concentrations of nanorose match values predicted from nanoparticle and material properties. Measurement of OP variation in rabbit arteries in response to laser excitation provides an estimate of nanorose concentration in atherosclerotic lesions of 2.5×l0⁹ particles/ml. OP variation in atherosclerotic lesions containing macrophages taking up nanorose has a different magnitude and profile from that observed in control thoracic aorta without macrophages and is consistent with macrophage presence as identified with RAM-11 histology staining. Our results suggest that tissue regions with macrophages taking up nanorose can be detected using photothermal OCT.

Original language	English (US)
Pages (from-to)	2-16
Number of pages	15
Journal	Biomedical Optics Express
Volume	1
Issue number	1
DOIs	https://doi.org/10.1364/BOE.1.000002
State	Published - Aug 2 2010
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Atomic and Molecular Physics, and Optics

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title = "Depth resolved photothermal OCT detection of macrophages in tissue using nanorose",

abstract = "Application of photothermal Optical Coherence Tomography (OCT) to detect macrophages in ex vivo rabbit arteries which have engulfed nanoclusters of gold coated iron oxide (nanorose) is reported. Nanorose engulfed by macrophages associated with atherosclerotic lesions in rabbit arteries absorb incident laser (800nm) energy and cause optical pathlength (OP) variation which is measured using photothermal OCT. OP variation in polydimethyl siloxane tissue phantoms containing varying concentrations of nanorose match values predicted from nanoparticle and material properties. Measurement of OP variation in rabbit arteries in response to laser excitation provides an estimate of nanorose concentration in atherosclerotic lesions of 2.5×l09 particles/ml. OP variation in atherosclerotic lesions containing macrophages taking up nanorose has a different magnitude and profile from that observed in control thoracic aorta without macrophages and is consistent with macrophage presence as identified with RAM-11 histology staining. Our results suggest that tissue regions with macrophages taking up nanorose can be detected using photothermal OCT.",

author = "Paranjape, {Amit S.} and Roman Kuranov and Stepan Baranov and Ma, {Li Leo} and Villard, {Joseph W.} and Tianyi Wang and Sokolov, {Konstantin V.} and Feldman, {Marc D.} and Johnston, {Keith P.} and Minier, {Thomas E.}",

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doi = "10.1364/BOE.1.000002",

language = "English (US)",

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AU - Paranjape, Amit S.

AU - Kuranov, Roman

AU - Baranov, Stepan

AU - Ma, Li Leo

AU - Villard, Joseph W.

AU - Wang, Tianyi

AU - Sokolov, Konstantin V.

AU - Feldman, Marc D.

AU - Johnston, Keith P.

AU - Minier, Thomas E.

PY - 2010/8/2

Y1 - 2010/8/2

N2 - Application of photothermal Optical Coherence Tomography (OCT) to detect macrophages in ex vivo rabbit arteries which have engulfed nanoclusters of gold coated iron oxide (nanorose) is reported. Nanorose engulfed by macrophages associated with atherosclerotic lesions in rabbit arteries absorb incident laser (800nm) energy and cause optical pathlength (OP) variation which is measured using photothermal OCT. OP variation in polydimethyl siloxane tissue phantoms containing varying concentrations of nanorose match values predicted from nanoparticle and material properties. Measurement of OP variation in rabbit arteries in response to laser excitation provides an estimate of nanorose concentration in atherosclerotic lesions of 2.5×l09 particles/ml. OP variation in atherosclerotic lesions containing macrophages taking up nanorose has a different magnitude and profile from that observed in control thoracic aorta without macrophages and is consistent with macrophage presence as identified with RAM-11 histology staining. Our results suggest that tissue regions with macrophages taking up nanorose can be detected using photothermal OCT.

AB - Application of photothermal Optical Coherence Tomography (OCT) to detect macrophages in ex vivo rabbit arteries which have engulfed nanoclusters of gold coated iron oxide (nanorose) is reported. Nanorose engulfed by macrophages associated with atherosclerotic lesions in rabbit arteries absorb incident laser (800nm) energy and cause optical pathlength (OP) variation which is measured using photothermal OCT. OP variation in polydimethyl siloxane tissue phantoms containing varying concentrations of nanorose match values predicted from nanoparticle and material properties. Measurement of OP variation in rabbit arteries in response to laser excitation provides an estimate of nanorose concentration in atherosclerotic lesions of 2.5×l09 particles/ml. OP variation in atherosclerotic lesions containing macrophages taking up nanorose has a different magnitude and profile from that observed in control thoracic aorta without macrophages and is consistent with macrophage presence as identified with RAM-11 histology staining. Our results suggest that tissue regions with macrophages taking up nanorose can be detected using photothermal OCT.

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Depth resolved photothermal OCT detection of macrophages in tissue using nanorose

Abstract

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