Design and radio-synthesis of somatostatin receptors targeted 68Ga-DOTA-Benereotide for non-invasive PET imaging

Fei Liu; Hua Zhu; Chuangui Li; Xinfeng Lin; Chiyi Xiong; Chun Li; Zhi Yang

doi:10.1007/s10967-015-4241-z

Design and radio-synthesis of somatostatin receptors targeted ⁶⁸Ga-DOTA-Benereotide for non-invasive PET imaging

Fei Liu, Hua Zhu, Chuangui Li, Xinfeng Lin, Chiyi Xiong, Chun Li, Zhi Yang

Cancer Systems Imaging

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Here we reported the synthesis, radiolabeling and evaluation of a novel positron emission tomography (PET) tracer DOTA-Benerotide labeled with ⁶⁸Ga with potential application in SSTR_1,2,3,5 positive tumors for PET imaging. Radiolabeling of DOTA-Benerotide with ⁶⁸Ga achieved its highest efficiency at pH 3.5 and 100 °C for 20 min. It also showed excellent in vitro stability in different buffers. Micro-PET imaging of human colorectal adenocarcinoma tumor cell (HT-29) bearing mice clearly delineated tumors at 4 h after tail vein injection of ⁶⁸Ga-DOTA-Benerotide tracer. Further studies are needed to identify the mechanism of cell/organ uptake of this new SSTR PET imaging tracer.

Original language	English (US)
Pages (from-to)	1069-1075
Number of pages	7
Journal	Journal of Radioanalytical and Nuclear Chemistry
Volume	307
Issue number	2
DOIs	https://doi.org/10.1007/s10967-015-4241-z
State	Published - Feb 1 2016

Keywords

Benerotide peptide
Ga tracer
Micro-PET imaging
Somatostatin receptors

ASJC Scopus subject areas

Analytical Chemistry
Nuclear Energy and Engineering
Radiology Nuclear Medicine and imaging
Pollution
Spectroscopy
Public Health, Environmental and Occupational Health
Health, Toxicology and Mutagenesis

Access to Document

10.1007/s10967-015-4241-z

Cite this

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title = "Design and radio-synthesis of somatostatin receptors targeted 68Ga-DOTA-Benereotide for non-invasive PET imaging",

abstract = "Here we reported the synthesis, radiolabeling and evaluation of a novel positron emission tomography (PET) tracer DOTA-Benerotide labeled with 68Ga with potential application in SSTR1,2,3,5 positive tumors for PET imaging. Radiolabeling of DOTA-Benerotide with 68Ga achieved its highest efficiency at pH 3.5 and 100 °C for 20 min. It also showed excellent in vitro stability in different buffers. Micro-PET imaging of human colorectal adenocarcinoma tumor cell (HT-29) bearing mice clearly delineated tumors at 4 h after tail vein injection of 68Ga-DOTA-Benerotide tracer. Further studies are needed to identify the mechanism of cell/organ uptake of this new SSTR PET imaging tracer.",

keywords = "Benerotide peptide, Ga tracer, Micro-PET imaging, Somatostatin receptors",

author = "Fei Liu and Hua Zhu and Chuangui Li and Xinfeng Lin and Chiyi Xiong and Chun Li and Zhi Yang",

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T1 - Design and radio-synthesis of somatostatin receptors targeted 68Ga-DOTA-Benereotide for non-invasive PET imaging

AU - Liu, Fei

AU - Zhu, Hua

AU - Li, Chuangui

AU - Lin, Xinfeng

AU - Xiong, Chiyi

AU - Li, Chun

AU - Yang, Zhi

PY - 2016/2/1

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AB - Here we reported the synthesis, radiolabeling and evaluation of a novel positron emission tomography (PET) tracer DOTA-Benerotide labeled with 68Ga with potential application in SSTR1,2,3,5 positive tumors for PET imaging. Radiolabeling of DOTA-Benerotide with 68Ga achieved its highest efficiency at pH 3.5 and 100 °C for 20 min. It also showed excellent in vitro stability in different buffers. Micro-PET imaging of human colorectal adenocarcinoma tumor cell (HT-29) bearing mice clearly delineated tumors at 4 h after tail vein injection of 68Ga-DOTA-Benerotide tracer. Further studies are needed to identify the mechanism of cell/organ uptake of this new SSTR PET imaging tracer.

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KW - Ga tracer

KW - Micro-PET imaging

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