TY - JOUR
T1 - Design and validation of a bifunctional ligand display system for receptor targeting
AU - Chen, Limor
AU - Zurita, Amado J.
AU - Ardelt, Peter U.
AU - Giordano, Ricardo J.
AU - Arap, Wadih
AU - Pasqualini, Renata
N1 - Funding Information:
We thank Dr. David Needleman for technical assistance and Drs. David LaVan and George P. Smith for advice and comments on an earlier version of the manuscript. This work was supported by National Institutes of Health grants CA90270 (to R.P. and W.A.), CA82976, CA78512, and CA88106 (to R.P.), and CA103042 and CA90810 (to W.A.), National Cancer Institute R01-DK67683 (to W.A.), Department of Defense DAMD17-03-1-0384 (to R.P.), and by awards from the Gilson-Longenbaugh Foundation, the V Foundation, and AngelWorks (all to R.P. and W.A.). A.J.Z. is the recipient of a fellowship from the Instituto de Salud Carlos III, Spain (BEFI 01/9526).
PY - 2004/8
Y1 - 2004/8
N2 - Here we developed a bacteriophage display particle designed to serve as a bifunctional entity that can target tumors while delivering an agent. We engineered a chimera phage vector containing a pIII-displayed αv integrins-targeting moiety and a pVIII-displayed streptavidin binding adaptor moiety. By using the chimeric phage particle, targeting of αv integrins on cells in culture and tumor-related blood vessels was shown through different applications, including luminescent quantum dots localization, surface plasmon resonance-based binding detection, and an in vivo tumor model. The strategy validated here will accelerate the discovery and characterization of receptor-ligand binding events in high throughput, and cell-specific delivery of diagnostics or therapeutics to organs of choice without the need for chemical conjugation.
AB - Here we developed a bacteriophage display particle designed to serve as a bifunctional entity that can target tumors while delivering an agent. We engineered a chimera phage vector containing a pIII-displayed αv integrins-targeting moiety and a pVIII-displayed streptavidin binding adaptor moiety. By using the chimeric phage particle, targeting of αv integrins on cells in culture and tumor-related blood vessels was shown through different applications, including luminescent quantum dots localization, surface plasmon resonance-based binding detection, and an in vivo tumor model. The strategy validated here will accelerate the discovery and characterization of receptor-ligand binding events in high throughput, and cell-specific delivery of diagnostics or therapeutics to organs of choice without the need for chemical conjugation.
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U2 - 10.1016/j.chembiol.2004.05.019
DO - 10.1016/j.chembiol.2004.05.019
M3 - Article
C2 - 15324809
AN - SCOPUS:4344616983
SN - 1074-5521
VL - 11
SP - 1081
EP - 1091
JO - Chemistry and Biology
JF - Chemistry and Biology
IS - 8
ER -