Detection of Alkaline Phosphatase Enzyme Activity with a CatalyCEST MRI Biosensor

Iman Daryaei; Mahsa Mohammadebrahim Ghaffari; Kyle M. Jones; Mark D. Pagel

doi:10.1021/acssensors.6b00203

Detection of Alkaline Phosphatase Enzyme Activity with a CatalyCEST MRI Biosensor

Iman Daryaei, Mahsa Mohammadebrahim Ghaffari, Kyle M. Jones, Mark D. Pagel

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

Responsive CEST MRI biosensors offer good sensitivity and excellent specificity for detection of biomarkers with great potential for clinical translation. We report the application of fosfosal, a phosphorylated form of salicylic acid, for the detection of alkaline phosphatase (AP) enzyme. We detected conversion of fosfosal to salicylic acid in the presence of the enzyme by CEST MRI. Importantly the technique was able to detect AP enzyme expressed in cells in the presence of other cell components, which improves specificity. Various isoforms of the enzyme showed different Michaelis-Menten kinetics and yet these kinetics studies indicated very efficient catalytic rates. Our results with the fosfosal biosensor encourage further in vivo studies.

Original language	English (US)
Pages (from-to)	857-861
Number of pages	5
Journal	ACS Sensors
Volume	1
Issue number	7
DOIs	https://doi.org/10.1021/acssensors.6b00203
State	Published - Jul 22 2016
Externally published	Yes

Keywords

CATALYCEST
CEST MRI
Michaelis-Menten kinetics
alkaline phosphatase
molecular imaging

ASJC Scopus subject areas

Bioengineering
Instrumentation
Process Chemistry and Technology
Fluid Flow and Transfer Processes

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10.1021/acssensors.6b00203

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title = "Detection of Alkaline Phosphatase Enzyme Activity with a CatalyCEST MRI Biosensor",

abstract = "Responsive CEST MRI biosensors offer good sensitivity and excellent specificity for detection of biomarkers with great potential for clinical translation. We report the application of fosfosal, a phosphorylated form of salicylic acid, for the detection of alkaline phosphatase (AP) enzyme. We detected conversion of fosfosal to salicylic acid in the presence of the enzyme by CEST MRI. Importantly the technique was able to detect AP enzyme expressed in cells in the presence of other cell components, which improves specificity. Various isoforms of the enzyme showed different Michaelis-Menten kinetics and yet these kinetics studies indicated very efficient catalytic rates. Our results with the fosfosal biosensor encourage further in vivo studies.",

keywords = "CATALYCEST, CEST MRI, Michaelis-Menten kinetics, alkaline phosphatase, molecular imaging",

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doi = "10.1021/acssensors.6b00203",

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AU - Daryaei, Iman

AU - Mohammadebrahim Ghaffari, Mahsa

AU - Jones, Kyle M.

AU - Pagel, Mark D.

PY - 2016/7/22

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N2 - Responsive CEST MRI biosensors offer good sensitivity and excellent specificity for detection of biomarkers with great potential for clinical translation. We report the application of fosfosal, a phosphorylated form of salicylic acid, for the detection of alkaline phosphatase (AP) enzyme. We detected conversion of fosfosal to salicylic acid in the presence of the enzyme by CEST MRI. Importantly the technique was able to detect AP enzyme expressed in cells in the presence of other cell components, which improves specificity. Various isoforms of the enzyme showed different Michaelis-Menten kinetics and yet these kinetics studies indicated very efficient catalytic rates. Our results with the fosfosal biosensor encourage further in vivo studies.

AB - Responsive CEST MRI biosensors offer good sensitivity and excellent specificity for detection of biomarkers with great potential for clinical translation. We report the application of fosfosal, a phosphorylated form of salicylic acid, for the detection of alkaline phosphatase (AP) enzyme. We detected conversion of fosfosal to salicylic acid in the presence of the enzyme by CEST MRI. Importantly the technique was able to detect AP enzyme expressed in cells in the presence of other cell components, which improves specificity. Various isoforms of the enzyme showed different Michaelis-Menten kinetics and yet these kinetics studies indicated very efficient catalytic rates. Our results with the fosfosal biosensor encourage further in vivo studies.

KW - CATALYCEST

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KW - Michaelis-Menten kinetics

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Detection of Alkaline Phosphatase Enzyme Activity with a CatalyCEST MRI Biosensor

Abstract

Keywords

ASJC Scopus subject areas

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