TY - JOUR
T1 - Detection of epstein-barr virus in the L and H cells of nodular lymphocyte predominance Hodgkin's disease
T2 - Report of a case documented by immunohistochemical, in situ hybridization, and polymerase chain reaction methods
AU - Khalidi, Hasan S.
AU - Lones, Mark A.
AU - Zhou, Yuehua
AU - Weiss, Lawrence M.
AU - Medeiros, L. Jeffrey
PY - 1997/12
Y1 - 1997/12
N2 - Although Epstein-Barr virus (EBV) is commonly present in the neoplastic Reed-Sternberg and Hodgkin cells of the mixed cellularity, nodular sclerosis, and lymphocyte depletion types of Hodgkin's disease (HD), EBV is rare in the neoplastic cells of the nodular lymphocyte predominance type of HD, particularly in the United States. We describe a 19-year-old Hispanic man in whom nodular lymphocyte predominance HD involved a cervical lymph node. Epstein-Barr virus was identified in the neoplastic L and H cells by using in situ hybridization for EBV RNA and immunohistochemical staining for EBV latent membrane protein-1. Polymerase chain reaction studies demonstrated the type A strain of EBV. The presence of EBV in this case may be related to drainage of the virus from the oropharynx to the cervical lymph node. The presence of EBV also may be related to this patient's Hispanic ethnic origin.
AB - Although Epstein-Barr virus (EBV) is commonly present in the neoplastic Reed-Sternberg and Hodgkin cells of the mixed cellularity, nodular sclerosis, and lymphocyte depletion types of Hodgkin's disease (HD), EBV is rare in the neoplastic cells of the nodular lymphocyte predominance type of HD, particularly in the United States. We describe a 19-year-old Hispanic man in whom nodular lymphocyte predominance HD involved a cervical lymph node. Epstein-Barr virus was identified in the neoplastic L and H cells by using in situ hybridization for EBV RNA and immunohistochemical staining for EBV latent membrane protein-1. Polymerase chain reaction studies demonstrated the type A strain of EBV. The presence of EBV in this case may be related to drainage of the virus from the oropharynx to the cervical lymph node. The presence of EBV also may be related to this patient's Hispanic ethnic origin.
KW - Epstein-Barr virus
KW - Hodgkin's disease
KW - Immunohistochemistry
KW - In situ hybridization
KW - Nodular lymphocyte predominance
KW - Polymerase chain reaction
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U2 - 10.1093/ajcp/108.6.687
DO - 10.1093/ajcp/108.6.687
M3 - Article
C2 - 9384451
AN - SCOPUS:0030724887
SN - 0002-9173
VL - 108
SP - 687
EP - 692
JO - American journal of clinical pathology
JF - American journal of clinical pathology
IS - 6
ER -