TY - CHAP
T1 - Detection of protein-protein interactions in live cells and animals with split firefly luciferase protein fragment complementation
AU - Villalobos, Victor
AU - Naik, Snehal
AU - Piwnica-Worms, David
PY - 2008
Y1 - 2008
N2 - Protein fragment complementation has emerged as a powerful tool for measuring protein-protein interactions in the context of live cells. The adaptation of this strategy for use with firefly luciferase now allows for the non-invasive, quantitative, real-time readout of protein interactions in lysates, live cells, and whole animals. Bioluminescence provides a robust imaging modality due to its extremely low background signal and large dynamic range. The split luciferase fusion constructs described here are inducible by addition of ligands, small molecules or drugs, in this example, rapamycin, and have been shown to work in vivo.
AB - Protein fragment complementation has emerged as a powerful tool for measuring protein-protein interactions in the context of live cells. The adaptation of this strategy for use with firefly luciferase now allows for the non-invasive, quantitative, real-time readout of protein interactions in lysates, live cells, and whole animals. Bioluminescence provides a robust imaging modality due to its extremely low background signal and large dynamic range. The split luciferase fusion constructs described here are inducible by addition of ligands, small molecules or drugs, in this example, rapamycin, and have been shown to work in vivo.
KW - Bioluminescence
KW - Firefly luciferase
KW - Molecular imaging
KW - Protein fragment complementation
KW - Protein-protein interactions
KW - Split luciferase complementation
UR - http://www.scopus.com/inward/record.url?scp=84934443674&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84934443674&partnerID=8YFLogxK
U2 - 10.1007/978-1-59745-188-8_23
DO - 10.1007/978-1-59745-188-8_23
M3 - Chapter
C2 - 18370114
AN - SCOPUS:84934443674
SN - 9781588298713
T3 - Methods in Molecular Biology
SP - 339
EP - 352
BT - Genomics Protocols
PB - Humana Press
ER -