Detection of sialic acid residues and studies of their organization in normal and tumor α1-acid glycoproteins as probed by surface-enhanced Raman spectroscopy

Konstantin V. Sokolov; Nargiz E. Byramova; Larisa V. Mochalova; Alexander B. Tuzikov; Svetlana D. Shiyan; Nicolai V. Bovin; Igor R. Nabiev

doi:10.1366/0003702934067153

Detection of sialic acid residues and studies of their organization in normal and tumor α₁-acid glycoproteins as probed by surface-enhanced Raman spectroscopy

Konstantin V. Sokolov, Nargiz E. Byramova, Larisa V. Mochalova, Alexander B. Tuzikov, Svetlana D. Shiyan, Nicolai V. Bovin, Igor R. Nabiev

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Surface-enhanced Raman scattering (SERS) spectra of sialic acid (SA) benzyl, and methyl glycosides as well as natural sialylated glycoproteins from human cells of healthy donors and tumor patients have been analyzed in view of the fact that peripheral fragments of many bioactive glycoconjugates are SA residues. SA residues can be detected by SERS at concentrations as low as 10^-6 M. The pattern of interaction of SA with the surface of a silver hydrosol involves the C-8 and C-9 hydroxy groups of SA. SERS spectroscopy is sensitive to changes in the content and type of branching of sialylated sugar chains in sialylglycoproteins. The differences in sialylated sugar-chain organization for α₁-acid glycoproteins from healthy donors and tumor patients have been detected by means of SERS spectroscopy. The first example of the detection of SA residues for a suspension of living cells has been presented.

Original language	English (US)
Pages (from-to)	535-538
Number of pages	4
Journal	Applied Spectroscopy
Volume	47
Issue number	5
DOIs	https://doi.org/10.1366/0003702934067153
State	Published - 1993
Externally published	Yes

Keywords

Glycoconjugates
SERS
Sialic acid

ASJC Scopus subject areas

Instrumentation
Spectroscopy

Access to Document

10.1366/0003702934067153

Cite this

@article{7d45d8eeafc545e68c5465d4c4d9ebba,

title = "Detection of sialic acid residues and studies of their organization in normal and tumor α1-acid glycoproteins as probed by surface-enhanced Raman spectroscopy",

abstract = "Surface-enhanced Raman scattering (SERS) spectra of sialic acid (SA) benzyl, and methyl glycosides as well as natural sialylated glycoproteins from human cells of healthy donors and tumor patients have been analyzed in view of the fact that peripheral fragments of many bioactive glycoconjugates are SA residues. SA residues can be detected by SERS at concentrations as low as 10-6 M. The pattern of interaction of SA with the surface of a silver hydrosol involves the C-8 and C-9 hydroxy groups of SA. SERS spectroscopy is sensitive to changes in the content and type of branching of sialylated sugar chains in sialylglycoproteins. The differences in sialylated sugar-chain organization for α1-acid glycoproteins from healthy donors and tumor patients have been detected by means of SERS spectroscopy. The first example of the detection of SA residues for a suspension of living cells has been presented.",

keywords = "Glycoconjugates, SERS, Sialic acid",

author = "Sokolov, {Konstantin V.} and Byramova, {Nargiz E.} and Mochalova, {Larisa V.} and Tuzikov, {Alexander B.} and Shiyan, {Svetlana D.} and Bovin, {Nicolai V.} and Nabiev, {Igor R.}",

year = "1993",

doi = "10.1366/0003702934067153",

language = "English (US)",

volume = "47",

pages = "535--538",

journal = "Applied Spectroscopy",

issn = "0003-7028",

publisher = "Society for Applied Spectroscopy",

number = "5",

}

TY - JOUR

T1 - Detection of sialic acid residues and studies of their organization in normal and tumor α1-acid glycoproteins as probed by surface-enhanced Raman spectroscopy

AU - Sokolov, Konstantin V.

AU - Byramova, Nargiz E.

AU - Mochalova, Larisa V.

AU - Tuzikov, Alexander B.

AU - Shiyan, Svetlana D.

AU - Bovin, Nicolai V.

AU - Nabiev, Igor R.

PY - 1993

Y1 - 1993

N2 - Surface-enhanced Raman scattering (SERS) spectra of sialic acid (SA) benzyl, and methyl glycosides as well as natural sialylated glycoproteins from human cells of healthy donors and tumor patients have been analyzed in view of the fact that peripheral fragments of many bioactive glycoconjugates are SA residues. SA residues can be detected by SERS at concentrations as low as 10-6 M. The pattern of interaction of SA with the surface of a silver hydrosol involves the C-8 and C-9 hydroxy groups of SA. SERS spectroscopy is sensitive to changes in the content and type of branching of sialylated sugar chains in sialylglycoproteins. The differences in sialylated sugar-chain organization for α1-acid glycoproteins from healthy donors and tumor patients have been detected by means of SERS spectroscopy. The first example of the detection of SA residues for a suspension of living cells has been presented.

AB - Surface-enhanced Raman scattering (SERS) spectra of sialic acid (SA) benzyl, and methyl glycosides as well as natural sialylated glycoproteins from human cells of healthy donors and tumor patients have been analyzed in view of the fact that peripheral fragments of many bioactive glycoconjugates are SA residues. SA residues can be detected by SERS at concentrations as low as 10-6 M. The pattern of interaction of SA with the surface of a silver hydrosol involves the C-8 and C-9 hydroxy groups of SA. SERS spectroscopy is sensitive to changes in the content and type of branching of sialylated sugar chains in sialylglycoproteins. The differences in sialylated sugar-chain organization for α1-acid glycoproteins from healthy donors and tumor patients have been detected by means of SERS spectroscopy. The first example of the detection of SA residues for a suspension of living cells has been presented.

KW - Glycoconjugates

KW - SERS

KW - Sialic acid

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DO - 10.1366/0003702934067153

M3 - Article

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SN - 0003-7028

VL - 47

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EP - 538

JO - Applied Spectroscopy

JF - Applied Spectroscopy

IS - 5

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