Detection of tumor‐associated antigen in human melanoma cell line supernatants

Elizabeth A. Grimm; Hulbert K.B. Silver; Jack A. Roth; Darwin O. Chee; Rishab K. Gupta; Donald L. Morton

doi:10.1002/ijc.2910170503

Detection of tumor‐associated antigen in human melanoma cell line supernatants

Elizabeth A. Grimm, Hulbert K.B. Silver, Jack A. Roth, Darwin O. Chee, Rishab K. Gupta, Donald L. Morton

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38 Scopus citations

Abstract

Spent tissue culture medium (CDM‐S) removed from a single cell line of human malignant melanoma grown in serum‐free CDM, contained tumor‐associated antigenic activity. Antibodies to CDM‐S measured by complement fixation were detected in 44% (31/70) melanoma, 55% (15/27) sarcoma, 63% (24/38) carcinoma and 15% (11/72) normal sera. Delayed cutaneous hypersensitivity reactions (DCHR) were demonstrated in 4/5 melanoma patients at a 500 μg dose, 3/5 at a 100 μg dose and in 1/7 carcinoma patients at the 500 μg dose. One ml of CDM‐S was shown to contain antigen equivalent to that obtained from the membranes of 2.9 × 10⁷ tissue‐cultured melanoma cells. After purification, 84% (16/19) sera from melanoma patients, 66% (12/18) from sarcoma and carcinoma patients and 8% (2/26) from normal controls were positive to the antigen by complement fixation.

Original language	English (US)
Pages (from-to)	559-564
Number of pages	6
Journal	International journal of cancer
Volume	17
Issue number	5
DOIs	https://doi.org/10.1002/ijc.2910170503
State	Published - May 15 1976

ASJC Scopus subject areas

Oncology
Cancer Research

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title = "Detection of tumor‐associated antigen in human melanoma cell line supernatants",

abstract = "Spent tissue culture medium (CDM‐S) removed from a single cell line of human malignant melanoma grown in serum‐free CDM, contained tumor‐associated antigenic activity. Antibodies to CDM‐S measured by complement fixation were detected in 44% (31/70) melanoma, 55% (15/27) sarcoma, 63% (24/38) carcinoma and 15% (11/72) normal sera. Delayed cutaneous hypersensitivity reactions (DCHR) were demonstrated in 4/5 melanoma patients at a 500 μg dose, 3/5 at a 100 μg dose and in 1/7 carcinoma patients at the 500 μg dose. One ml of CDM‐S was shown to contain antigen equivalent to that obtained from the membranes of 2.9 × 107 tissue‐cultured melanoma cells. After purification, 84% (16/19) sera from melanoma patients, 66% (12/18) from sarcoma and carcinoma patients and 8% (2/26) from normal controls were positive to the antigen by complement fixation.",

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T1 - Detection of tumor‐associated antigen in human melanoma cell line supernatants

AU - Grimm, Elizabeth A.

AU - Silver, Hulbert K.B.

AU - Roth, Jack A.

AU - Chee, Darwin O.

AU - Gupta, Rishab K.

AU - Morton, Donald L.

PY - 1976/5/15

Y1 - 1976/5/15

N2 - Spent tissue culture medium (CDM‐S) removed from a single cell line of human malignant melanoma grown in serum‐free CDM, contained tumor‐associated antigenic activity. Antibodies to CDM‐S measured by complement fixation were detected in 44% (31/70) melanoma, 55% (15/27) sarcoma, 63% (24/38) carcinoma and 15% (11/72) normal sera. Delayed cutaneous hypersensitivity reactions (DCHR) were demonstrated in 4/5 melanoma patients at a 500 μg dose, 3/5 at a 100 μg dose and in 1/7 carcinoma patients at the 500 μg dose. One ml of CDM‐S was shown to contain antigen equivalent to that obtained from the membranes of 2.9 × 107 tissue‐cultured melanoma cells. After purification, 84% (16/19) sera from melanoma patients, 66% (12/18) from sarcoma and carcinoma patients and 8% (2/26) from normal controls were positive to the antigen by complement fixation.

AB - Spent tissue culture medium (CDM‐S) removed from a single cell line of human malignant melanoma grown in serum‐free CDM, contained tumor‐associated antigenic activity. Antibodies to CDM‐S measured by complement fixation were detected in 44% (31/70) melanoma, 55% (15/27) sarcoma, 63% (24/38) carcinoma and 15% (11/72) normal sera. Delayed cutaneous hypersensitivity reactions (DCHR) were demonstrated in 4/5 melanoma patients at a 500 μg dose, 3/5 at a 100 μg dose and in 1/7 carcinoma patients at the 500 μg dose. One ml of CDM‐S was shown to contain antigen equivalent to that obtained from the membranes of 2.9 × 107 tissue‐cultured melanoma cells. After purification, 84% (16/19) sera from melanoma patients, 66% (12/18) from sarcoma and carcinoma patients and 8% (2/26) from normal controls were positive to the antigen by complement fixation.

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Detection of tumor‐associated antigen in human melanoma cell line supernatants

Abstract

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