Abstract
A new method of chemiluminescent enzyme immunoassay (CLEIA) was developed and the standard curve and regression equation for determination of progesterone receptor (PR) made. The luminosity of tissue samples was tested and PR level was calculated by the regression equation. Correlation analysis revealed that there was a linear relationship between different concentrations of the standard PR samples and the corresponding values of luminosity: Y = 3748+463.77X, γ = 0.9958. The values of the luminosity in 38 cases of tumor tissues were determined with the highest being 267.32 fmol/mg, the lowest 3.69 fmol/mg and the mean 78.53 fmol/mg. The new method of CLEIA was a stable, creditable, specific and sensitive assay for determination of PR.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 60-61 |
| Number of pages | 2 |
| Journal | Journal of Huazhong University of Science and Technology - Medical Science |
| Volume | 23 |
| Issue number | 1 |
| State | Published - 2003 |
| Externally published | Yes |
Keywords
- Chemiluminescent enzyme immunoassay
- Progesterone receptor
ASJC Scopus subject areas
- Biochemistry
- Biomaterials
- Biomedical Engineering
- Genetics