Abstract
It has been shown that the monoclonal antibody Ki-67 reacts with a nuclear antigen that is expressed only by proliferating cells. The feasibility of using image analysis to quantitate Ki-67 staining (proliferation index [PI]) of epithelial ovarian cancers was investigated. The PI was determined in 50 advanced-stage primary ovarian cancers. Frozen sections were immunostained with the Ki-67 monoclonal antibody, and the PI was calculated using static image analysis. Among 35 stage III ovarian carcinomas, the median PI was 8.9%, compared with 17.7% in 15 stage IV cancers (P = 0.06). There was no relationship between PI and histologic grade. The median survival time of 32 patients whose cancers had a high Ki-67 expression (≥7.5%) was 16.8 months, which differed significantly (P <0.01) from the median survival of 31.5 months observed in patients whose tumors demonstrated low Ki-67 expression (<7.5%). Quantitative image analysis of Ki-67-stained fresh-frozen ovarian cancers may provide useful prognostic information. Further studies are warranted to investigate the relationship between Ki-67 expression and other known clinicopathologic and genetic features of ovarian cancer.
Original language | English (US) |
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Pages (from-to) | 736-740 |
Number of pages | 5 |
Journal | American journal of clinical pathology |
Volume | 99 |
Issue number | 6 |
DOIs | |
State | Published - 1993 |
Externally published | Yes |
Keywords
- Image cytometry
- Immunohistochemistry
- Ki-67
- Ovarian cancer
ASJC Scopus subject areas
- Pathology and Forensic Medicine