TY - JOUR
T1 - Determination of tamoxifen and metabolites in human serum by high-performance liquid chromatography with post-column fluorescence activation
AU - Brown, Raymond R.
AU - Bain, Richard
AU - Jordan, V. Craig
N1 - Funding Information:
Supported by USPHS grants POl-CA 20432 and P30-CA 14520 from the National Institutes of Health and funds from ICI Ltd., Pharmaceuticals Division, Macclesfield, Great Britain and Stuart Pharmaceuticals, Wilmington, DE, U.S.A. We are extremely grateful to Drs. A.H. Todd and H.K. Adam of ICI Ltd. for their helpful comments and advice during these studies.
PY - 1983
Y1 - 1983
N2 - Sensitive and reproducible analyses were developed for assaying tamoxifen, monohydroxytamoxifen, N-desmethyltamoxifen, metabolite E [trans-1(4-hydroxyphenyl)1,2-diphenylbut-1-ene] and a new metabolite, metabolite Y [trans-1(4-hydroxyethoxyphenyl)-1,2-diphenylbut-1-ene] in human serum using high-performance liquid chromatography (HPLC). Three different systems were developed for specific purposes. All chromatography was performed using serum extracts made with hexane-butanol. Detection was by fluorimetry of phenanthrene derivatives formed by on-stream UV irradiation with a newly described device for post-column irradiation of the HPLC stream. This device may be of use in other HPLC system requiring post-column photochemical reactions.
AB - Sensitive and reproducible analyses were developed for assaying tamoxifen, monohydroxytamoxifen, N-desmethyltamoxifen, metabolite E [trans-1(4-hydroxyphenyl)1,2-diphenylbut-1-ene] and a new metabolite, metabolite Y [trans-1(4-hydroxyethoxyphenyl)-1,2-diphenylbut-1-ene] in human serum using high-performance liquid chromatography (HPLC). Three different systems were developed for specific purposes. All chromatography was performed using serum extracts made with hexane-butanol. Detection was by fluorimetry of phenanthrene derivatives formed by on-stream UV irradiation with a newly described device for post-column irradiation of the HPLC stream. This device may be of use in other HPLC system requiring post-column photochemical reactions.
UR - http://www.scopus.com/inward/record.url?scp=0020676409&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0020676409&partnerID=8YFLogxK
U2 - 10.1016/S0378-4347(00)86138-1
DO - 10.1016/S0378-4347(00)86138-1
M3 - Article
C2 - 6833432
AN - SCOPUS:0020676409
SN - 0378-4347
VL - 272
SP - 351
EP - 358
JO - Journal of Chromatography B: Biomedical Sciences and Applications
JF - Journal of Chromatography B: Biomedical Sciences and Applications
IS - C
ER -