Development and characterization of salivary adenoid cystic carcinoma cell line

Jie Li; Laszlo Perlaky; Pulivarthi Rao; Randal S. Weber; Adel K. El-Naggar

doi:10.1016/j.oraloncology.2014.06.012

Development and characterization of salivary adenoid cystic carcinoma cell line

Jie Li, Laszlo Perlaky, Pulivarthi Rao, Randal S. Weber, Adel K. El-Naggar

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

Objective: To develop in vitro adenoid cystic carcinoma cell line as a surrogate for functional studies. Materials and methods Cells obtained from a primary ACC of the base of tongue were cultivated in vitro and immortalized with h-TERT. Morphologic, cytogenetic and functional studies were performed.

Results: Tumor cells were verified by positive reactions to keratin and smooth muscle actin and phenotypic cellular and nuclear features. In-vitro cell growth and colony formation assay supported their tumor nature.

Conclusion: We authenticated an ACC cell line with hybrid epithelial-myoepithelial feature as a resource for functional experimentation.

Original language	English (US)
Pages (from-to)	991-999
Number of pages	9
Journal	Oral Oncology
Volume	50
Issue number	10
DOIs	https://doi.org/10.1016/j.oraloncology.2014.06.012
State	Published - Oct 1 2014

Keywords

Adenoid cystic carcinoma
Cell line
Salivary gland tumors

ASJC Scopus subject areas

Oral Surgery
Oncology
Cancer Research

MD Anderson CCSG core facilities

Advanced Technology Genomics Core
Research Animal Support Facility
Tissue Biospecimen and Pathology Resource
Cytogenetics and Cell Authentication Core
Clinical Trials Office

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10.1016/j.oraloncology.2014.06.012

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title = "Development and characterization of salivary adenoid cystic carcinoma cell line",

abstract = "Objective: To develop in vitro adenoid cystic carcinoma cell line as a surrogate for functional studies. Materials and methods Cells obtained from a primary ACC of the base of tongue were cultivated in vitro and immortalized with h-TERT. Morphologic, cytogenetic and functional studies were performed.Results: Tumor cells were verified by positive reactions to keratin and smooth muscle actin and phenotypic cellular and nuclear features. In-vitro cell growth and colony formation assay supported their tumor nature.Conclusion: We authenticated an ACC cell line with hybrid epithelial-myoepithelial feature as a resource for functional experimentation.",

keywords = "Adenoid cystic carcinoma, Cell line, Salivary gland tumors",

author = "Jie Li and Laszlo Perlaky and Pulivarthi Rao and Weber, {Randal S.} and El-Naggar, {Adel K.}",

note = "Funding Information: The study is supported in part by the NIH National Institute of Dental and Craniofacial Research and the NIH Office of Rare Diseases Research Grant No. HHSN268200900039C, The Salivary Gland Tumor Bank (SGTB), The Kenneth D. Muller professorship and an NCI CA-16672 grant. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Cancer Institute or the National Institutes of Health. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Publisher Copyright: {\textcopyright} 2014 Elsevier Ltd. All rights reserved.",

year = "2014",

month = oct,

day = "1",

doi = "10.1016/j.oraloncology.2014.06.012",

language = "English (US)",

volume = "50",

pages = "991--999",

journal = "Oral Oncology",

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TY - JOUR

T1 - Development and characterization of salivary adenoid cystic carcinoma cell line

AU - Li, Jie

AU - Perlaky, Laszlo

AU - Rao, Pulivarthi

AU - Weber, Randal S.

AU - El-Naggar, Adel K.

N1 - Funding Information: The study is supported in part by the NIH National Institute of Dental and Craniofacial Research and the NIH Office of Rare Diseases Research Grant No. HHSN268200900039C, The Salivary Gland Tumor Bank (SGTB), The Kenneth D. Muller professorship and an NCI CA-16672 grant. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Cancer Institute or the National Institutes of Health. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Publisher Copyright: © 2014 Elsevier Ltd. All rights reserved.

PY - 2014/10/1

Y1 - 2014/10/1

N2 - Objective: To develop in vitro adenoid cystic carcinoma cell line as a surrogate for functional studies. Materials and methods Cells obtained from a primary ACC of the base of tongue were cultivated in vitro and immortalized with h-TERT. Morphologic, cytogenetic and functional studies were performed.Results: Tumor cells were verified by positive reactions to keratin and smooth muscle actin and phenotypic cellular and nuclear features. In-vitro cell growth and colony formation assay supported their tumor nature.Conclusion: We authenticated an ACC cell line with hybrid epithelial-myoepithelial feature as a resource for functional experimentation.

AB - Objective: To develop in vitro adenoid cystic carcinoma cell line as a surrogate for functional studies. Materials and methods Cells obtained from a primary ACC of the base of tongue were cultivated in vitro and immortalized with h-TERT. Morphologic, cytogenetic and functional studies were performed.Results: Tumor cells were verified by positive reactions to keratin and smooth muscle actin and phenotypic cellular and nuclear features. In-vitro cell growth and colony formation assay supported their tumor nature.Conclusion: We authenticated an ACC cell line with hybrid epithelial-myoepithelial feature as a resource for functional experimentation.

KW - Adenoid cystic carcinoma

KW - Cell line

KW - Salivary gland tumors

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AN - SCOPUS:84907879254

SN - 1368-8375

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JO - Oral Oncology

JF - Oral Oncology

IS - 10

ER -

Development and characterization of salivary adenoid cystic carcinoma cell line

Abstract

Keywords

ASJC Scopus subject areas

MD Anderson CCSG core facilities

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