TY - JOUR
T1 - Development of an integrated multimodal optical imaging system with real-time image analysis for the evaluation of oral premalignant lesions
AU - Yang, Eric C.
AU - Vohra, Imran S.
AU - Badaoui, Hawraa
AU - Schwarz, Richard A.
AU - Cherry, Katelin D.
AU - Quang, Timothy
AU - Jacob, Justin
AU - Lang, Alex
AU - Bass, Nancy
AU - Rodriguez, Jessica
AU - Williams, Michelle D.
AU - Vigneswaran, Nadarajah
AU - Gillenwater, Ann M.
AU - Richards-Kortum, Rebecca R.
N1 - Funding Information:
This work was supported by the National Institutes of Health Grant Nos. RO1 CA103830 (to R. Richards-Kortum); RO1 CA185207 (to R. Richards-Kortum); RO1 DE024392 (to N. Vigneswaran); F30CA213922 (to E. Yang), and by the Cancer Prevention and Research Institute of Texas (CPRIT) Grant No. RP100932 (to R. Richards-Kortum).
Publisher Copyright:
© 2049 The Authors.
PY - 2019/2/1
Y1 - 2019/2/1
N2 - Oral premalignant lesions (OPLs), such as leukoplakia, are at risk of malignant transformation to oral cancer. Clinicians can elect to biopsy OPLs and assess them for dysplasia, a marker of increased risk. However, it is challenging to decide which OPLs need a biopsy and to select a biopsy site. We developed a multimodal optical imaging system (MMIS) that fully integrates the acquisition, display, and analysis of macroscopic white-light (WL), autofluorescence (AF), and high-resolution microendoscopy (HRME) images to noninvasively evaluate OPLs. WL and AF images identify suspicious regions with high sensitivity, which are explored at higher resolution with the HRME to improve specificity. Key features include a heat map that delineates suspicious regions according to AF images, and real-time image analysis algorithms that predict pathologic diagnosis at imaged sites. Representative examples from ongoing studies of the MMIS demonstrate its ability to identify high-grade dysplasia in OPLs that are not clinically suspicious, and to avoid unnecessary biopsies of benign OPLs that are clinically suspicious. The MMIS successfully integrates optical imaging approaches (WL, AF, and HRME) at multiple scales for the noninvasive evaluation of OPLs.
AB - Oral premalignant lesions (OPLs), such as leukoplakia, are at risk of malignant transformation to oral cancer. Clinicians can elect to biopsy OPLs and assess them for dysplasia, a marker of increased risk. However, it is challenging to decide which OPLs need a biopsy and to select a biopsy site. We developed a multimodal optical imaging system (MMIS) that fully integrates the acquisition, display, and analysis of macroscopic white-light (WL), autofluorescence (AF), and high-resolution microendoscopy (HRME) images to noninvasively evaluate OPLs. WL and AF images identify suspicious regions with high sensitivity, which are explored at higher resolution with the HRME to improve specificity. Key features include a heat map that delineates suspicious regions according to AF images, and real-time image analysis algorithms that predict pathologic diagnosis at imaged sites. Representative examples from ongoing studies of the MMIS demonstrate its ability to identify high-grade dysplasia in OPLs that are not clinically suspicious, and to avoid unnecessary biopsies of benign OPLs that are clinically suspicious. The MMIS successfully integrates optical imaging approaches (WL, AF, and HRME) at multiple scales for the noninvasive evaluation of OPLs.
KW - autofluorescence
KW - fiber bundle
KW - in vivo imaging
KW - oral cancer
KW - oral lesion
UR - http://www.scopus.com/inward/record.url?scp=85061997165&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85061997165&partnerID=8YFLogxK
U2 - 10.1117/1.JBO.24.2.025003
DO - 10.1117/1.JBO.24.2.025003
M3 - Article
C2 - 30793567
AN - SCOPUS:85061997165
SN - 1083-3668
VL - 24
JO - Journal of biomedical optics
JF - Journal of biomedical optics
IS - 2
M1 - 025003
ER -