TY - GEN
T1 - Development of automated high throughput single molecular microfluidic detection platform for signal transduction analysis
AU - Huang, Po Jung
AU - Baghbani Kordmahale, Sina
AU - Chou, Chao Kai
AU - Yamaguchi, Hirohito
AU - Hung, Mien Chie
AU - Kameoka, Jun
N1 - Publisher Copyright:
© 2016 SPIE.
PY - 2016
Y1 - 2016
N2 - Signal transductions including multiple protein post-translational modifications (PTM), protein-protein interactions (PPI), and protein-nucleic acid interaction (PNI) play critical roles for cell proliferation and differentiation that are directly related to the cancer biology. Traditional methods, like mass spectrometry, immunoprecipitation, fluorescence resonance energy transfer, and fluorescence correlation spectroscopy require a large amount of sample and long processing time. "microchannel for multiple-parameter analysis of proteins in single-complex (mMAPS)"we proposed can reduce the process time and sample volume because this system is composed by microfluidic channels, fluorescence microscopy, and computerized data analysis. In this paper, we will present an automated mMAPS including integrated microfluidic device, automated stage and electrical relay for high-throughput clinical screening. Based on this result, we estimated that this automated detection system will be able to screen approximately 150 patient samples in a 24-hour period, providing a practical application to analyze tissue samples in a clinical setting.
AB - Signal transductions including multiple protein post-translational modifications (PTM), protein-protein interactions (PPI), and protein-nucleic acid interaction (PNI) play critical roles for cell proliferation and differentiation that are directly related to the cancer biology. Traditional methods, like mass spectrometry, immunoprecipitation, fluorescence resonance energy transfer, and fluorescence correlation spectroscopy require a large amount of sample and long processing time. "microchannel for multiple-parameter analysis of proteins in single-complex (mMAPS)"we proposed can reduce the process time and sample volume because this system is composed by microfluidic channels, fluorescence microscopy, and computerized data analysis. In this paper, we will present an automated mMAPS including integrated microfluidic device, automated stage and electrical relay for high-throughput clinical screening. Based on this result, we estimated that this automated detection system will be able to screen approximately 150 patient samples in a 24-hour period, providing a practical application to analyze tissue samples in a clinical setting.
KW - Microfluidic device
KW - automated system
KW - flow-proteomic
KW - high-throughput analysis
KW - protein-protein complex interaction
KW - single molecule detection
UR - http://www.scopus.com/inward/record.url?scp=84973866037&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84973866037&partnerID=8YFLogxK
U2 - 10.1117/12.2213259
DO - 10.1117/12.2213259
M3 - Conference contribution
AN - SCOPUS:84973866037
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Microfluidics, BioMEMS, and Medical Microsystems XIV
A2 - Becker, Holger
A2 - Gray, Bonnie L.
PB - SPIE
T2 - 14th SPIE Photonics West Conference: Microfluidics, BioMEMS, and Medical Microsystems
Y2 - 13 February 2016 through 15 February 2016
ER -