TY - JOUR
T1 - Dielectrophoresis-based sample handling in general-purpose programmable diagnostic instruments
AU - Gascoyne, Peter R.C.
AU - Vykoukal, Jody V.
N1 - Funding Information:
Manuscript received April 1, 2003; revised August 2, 2003. This work was supported in part by the Defense Advanced Research Projects Agency under Contract N66001-97-C-8608 and Grant DAAD19-00-1-0515 from the Army Research Office, in part by National Cancer Institute under Award 4R33 CA 88346-2, and in part by National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) under Award 5R01-DK56105-06.
PY - 2004/1
Y1 - 2004/1
N2 - As the molecular origins of disease are better understood, the need for affordable, rapid, and automated technologies that enable microscale molecular diagnostics has become apparent. Widespread use of microsystems that perform sample preparation and molecular analysis could ensure that the benefits of new biomedical discoveries are realized by a maximum number of people, even those in environments lacking any infrastructure. While progress has been made in developing miniaturized diagnostic systems, samples are generally processed off-device using labor-intensive and time-consuming traditional sample preparation methods. We present the concept of an integrated programmable general-purpose sample analysis processor (GSAP) architecture where raw samples are routed to separation and analysis functional blocks contained within a single device. Several dielectrophoresis-based methods that could serve as the foundation for building GSAP functional blocks are reviewed including methods for cell and particle sorting, cell focusing, cell ac impedance analysis, cell lysis, and the manipulation of molecules and reagent droplets.
AB - As the molecular origins of disease are better understood, the need for affordable, rapid, and automated technologies that enable microscale molecular diagnostics has become apparent. Widespread use of microsystems that perform sample preparation and molecular analysis could ensure that the benefits of new biomedical discoveries are realized by a maximum number of people, even those in environments lacking any infrastructure. While progress has been made in developing miniaturized diagnostic systems, samples are generally processed off-device using labor-intensive and time-consuming traditional sample preparation methods. We present the concept of an integrated programmable general-purpose sample analysis processor (GSAP) architecture where raw samples are routed to separation and analysis functional blocks contained within a single device. Several dielectrophoresis-based methods that could serve as the foundation for building GSAP functional blocks are reviewed including methods for cell and particle sorting, cell focusing, cell ac impedance analysis, cell lysis, and the manipulation of molecules and reagent droplets.
KW - Cell separation
KW - Dielectrophoresis
KW - Molecular diagnostics
KW - Sample preparation
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U2 - 10.1109/JPROC.2003.820535
DO - 10.1109/JPROC.2003.820535
M3 - Article
C2 - 19684877
AN - SCOPUS:4344645067
SN - 0018-9219
VL - 92
SP - 22
EP - 42
JO - Proceedings of the IEEE
JF - Proceedings of the IEEE
IS - 1
ER -