TY - JOUR
T1 - Differential human serologic response to two 60, 000 molecular weight chlamydia trachomatis antigens
AU - Wagar, Elizabeth A.
AU - Schachter, Julius
AU - Bavoil, Patrik
AU - Stephens, Richard S.
N1 - Funding Information:
Received 12 September 1989; revised 2 April 1990. Presented in part (abstract D28) at the 89th annual meeting of the American Society for Microbiology, May 1989, New Orleans. Informed consent was obtained from the patients or their parents or guardians. Guidelines for human experimentation of the US Department of Health and Human Services were followed in the conduct of the clinical research. Financial support: National Institutes of Health (EY-07757, AI-21912, -24768), Research to Prevent Blindness, Inc., and the John D. and Catherine T. MacArthur Foundation. Reprints and correspondence: Dr. Julius Schachter, San Francisco General Hospital, BLDG. 30, Room 416, San Francisco, CA 94110. * Present address: Department of Pathology and Laboratory Medicine, UCLA School of Medicine, Los Angeles, CA.
PY - 1990/10
Y1 - 1990/10
N2 - Chlamydia trachomatis causes sexually transmitted diseases and is associated with serious longterm sequelae such as tubal infertility and ectopic pregnancy. There have been suggestions that chlamydial antigens of ˜57, 000–60, 000 Mr may be involved in the immunopathology. Two important chlamydial antigens of 57, 000–60, 000 Mr are a Triton X-100–soluble antigen, which induces hypersensitivity in ocular models, and a sarcosyl-insoluble cysteine-rich structural protein, omp2. In this study, a 57, 000 Mr Triton X-100–soluble protein was characterized as the chlamydial homolog of groEL, a heat shock protein. Using protein fractions, antibody responses of pelvic inflammatory disease (PID) and ectopic pregnancy patients to chlamydial groEL and omp2 were differentiated. Nearly all patients in both groups were reactive to omp2.Of those with titers ≥1:512, 31% of PID sera and 81% of ectopic pregnancy sera were positive for chlamydial groEL (P =.004). This selectivity suggests that women with PID who develop chronic sequelae are those with antibody to groEL.
AB - Chlamydia trachomatis causes sexually transmitted diseases and is associated with serious longterm sequelae such as tubal infertility and ectopic pregnancy. There have been suggestions that chlamydial antigens of ˜57, 000–60, 000 Mr may be involved in the immunopathology. Two important chlamydial antigens of 57, 000–60, 000 Mr are a Triton X-100–soluble antigen, which induces hypersensitivity in ocular models, and a sarcosyl-insoluble cysteine-rich structural protein, omp2. In this study, a 57, 000 Mr Triton X-100–soluble protein was characterized as the chlamydial homolog of groEL, a heat shock protein. Using protein fractions, antibody responses of pelvic inflammatory disease (PID) and ectopic pregnancy patients to chlamydial groEL and omp2 were differentiated. Nearly all patients in both groups were reactive to omp2.Of those with titers ≥1:512, 31% of PID sera and 81% of ectopic pregnancy sera were positive for chlamydial groEL (P =.004). This selectivity suggests that women with PID who develop chronic sequelae are those with antibody to groEL.
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U2 - 10.1093/infdis/162.4.922
DO - 10.1093/infdis/162.4.922
M3 - Article
C2 - 2205652
AN - SCOPUS:0025001399
SN - 0022-1899
VL - 162
SP - 922
EP - 927
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 4
ER -