TY - JOUR
T1 - Differential sensitivity of CD4+ and CD8+ T lymphocytes to phorbol myristate acetate upon anti-CD3 stimulation
T2 - Evidence for a distinct signaling pathway
AU - Chouaib, S.
AU - Mahe, Y.
AU - Mechri, S.
AU - Andreeff, M.
AU - Welte, K.
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1991
Y1 - 1991
N2 - The effects of suboptimal concentrations of Phorbol Myristate Acetate (PMA) in combination with submitogenic concentrations of OKT3 antibody on CD4+ and CD8+ cell activation were investigated. Upon stimulation with OKT3 (25 pg/ml) and PMA (0.25-0.75 ng/ml), the majority of CD4+ cells entered the cell cycle whereas most of CD8 + cells remained in G0/G1 phase. Under the same conditions of OKT3 stimulations, both CD4+ and CD8+ cells failed to produce IL2 in the absence of PMA. In the presence of PMA (0,25 ng/ml), CD4+ produced measurable amounts of IL2 (0,5-2,3 U/ml) whereas IL2 production by CD8 + cells remained below the detection limit. Expression of TAC antigen (CD25) was found to parallel IL2 production and cell proliferation in both subsets whereas changes in [Ca2+] mobilization following OKT3 stimulation were similar in both subsets. Interestingly, the elevation of intracellular cyclic adenosine 3':5' monophosphate (cAMP) was not equally distributed between CD4+ and CD8+ subpopulations. Furthermore, the kinetics of protein kinase (PKC) translocation was markedly prolonged in membranes of CD4+ cells compared with CD8+ cells suggesting a differential involvement that may operate under distinct regulatory signaling mechanisms.
AB - The effects of suboptimal concentrations of Phorbol Myristate Acetate (PMA) in combination with submitogenic concentrations of OKT3 antibody on CD4+ and CD8+ cell activation were investigated. Upon stimulation with OKT3 (25 pg/ml) and PMA (0.25-0.75 ng/ml), the majority of CD4+ cells entered the cell cycle whereas most of CD8 + cells remained in G0/G1 phase. Under the same conditions of OKT3 stimulations, both CD4+ and CD8+ cells failed to produce IL2 in the absence of PMA. In the presence of PMA (0,25 ng/ml), CD4+ produced measurable amounts of IL2 (0,5-2,3 U/ml) whereas IL2 production by CD8 + cells remained below the detection limit. Expression of TAC antigen (CD25) was found to parallel IL2 production and cell proliferation in both subsets whereas changes in [Ca2+] mobilization following OKT3 stimulation were similar in both subsets. Interestingly, the elevation of intracellular cyclic adenosine 3':5' monophosphate (cAMP) was not equally distributed between CD4+ and CD8+ subpopulations. Furthermore, the kinetics of protein kinase (PKC) translocation was markedly prolonged in membranes of CD4+ cells compared with CD8+ cells suggesting a differential involvement that may operate under distinct regulatory signaling mechanisms.
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U2 - 10.1177/039463209100400103
DO - 10.1177/039463209100400103
M3 - Article
AN - SCOPUS:0025778844
SN - 0394-6320
VL - 4
SP - 19
EP - 32
JO - International Journal of Immunopathology and Pharmacology
JF - International Journal of Immunopathology and Pharmacology
IS - 1
ER -