TY - JOUR
T1 - Differential structured illumination microendoscopy for in vivo imaging of molecular contrast agents
AU - Keahey, Pelham
AU - Ramalingam, Preetha
AU - Schmeler, Kathleen
AU - Richards-Kortum, Rebecca R.
N1 - Funding Information:
We thank the clinical research support staff at MD Anderson for their tireless work and effort in supporting this work. Research reported in this publication was supported by National Cancer Institute, National Institutes of Health Grants R01CA140257, R01CA103830, and R01CA186132. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
PY - 2016/9/27
Y1 - 2016/9/27
N2 - Fiber optic microendoscopy has shown promise for visualization of molecular contrast agents used to study disease in vivo. However, fiber optic microendoscopes have limited optical sectioning capability, and image contrast is limited by out-of-focus light generated in highly scattering tissue. Optical sectioning techniques have been used in microendoscopes to remove out-of-focus light but reduce imaging speed or rely on bulky optical elements that prevent in vivo imaging. Here, we present differential structured illumination microendoscopy (DSIMe), a fiber optic system that can perform structured illumination in real time for optical sectioning without any opto-mechanical components attached to the distal tip of the fiber bundle. We demonstrate the use of DSIMe during in vivo fluorescence imaging in patients undergoing surgery for cervical adenocarcinoma in situ. Images acquired using DSIMe show greater contrast than standard microendoscopy, improving the ability to detect cellular atypia associated with neoplasia.
AB - Fiber optic microendoscopy has shown promise for visualization of molecular contrast agents used to study disease in vivo. However, fiber optic microendoscopes have limited optical sectioning capability, and image contrast is limited by out-of-focus light generated in highly scattering tissue. Optical sectioning techniques have been used in microendoscopes to remove out-of-focus light but reduce imaging speed or rely on bulky optical elements that prevent in vivo imaging. Here, we present differential structured illumination microendoscopy (DSIMe), a fiber optic system that can perform structured illumination in real time for optical sectioning without any opto-mechanical components attached to the distal tip of the fiber bundle. We demonstrate the use of DSIMe during in vivo fluorescence imaging in patients undergoing surgery for cervical adenocarcinoma in situ. Images acquired using DSIMe show greater contrast than standard microendoscopy, improving the ability to detect cellular atypia associated with neoplasia.
KW - Fluorescence
KW - Microendoscopy
KW - Molecular imaging|cervical cancer
KW - Structured illumination
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U2 - 10.1073/pnas.1613497113
DO - 10.1073/pnas.1613497113
M3 - Article
C2 - 27621464
AN - SCOPUS:84989911302
SN - 0027-8424
VL - 113
SP - 10769
EP - 10773
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 39
ER -