TY - JOUR
T1 - Differential toxic effect of cis-platinum(II) and palladium(II) chlorides complexed with methyl 3,4-diamine-2,3,4,6-tetradeoxy-α-L-lyxo-hexopyranoside in mouse lymphoma cell lines differing in DSB and NER repair ability
AU - Kruszewski, Marcin
AU - Bouzyk, Elzbieta
AU - Oldak, Tomasz
AU - Samochocka, Krystyna
AU - Fuks, Leon
AU - Lewandowski, Włodzimierz
AU - Fokt, Izabela
AU - Priebe, Waldemar
N1 - Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2003
Y1 - 2003
N2 - The aim of this work was to test the cytotoxicity of newly synthesized cis-type complexes of platinum(II) and palladium(II) dichloride with methyl 3,4-diamine-2,3,4,6-tetradeoxy-α-L-lyxohexopyranoside, [M(C7H16 N2O2)Cl2]·H2O, against two mouse lymphoma cell lines (L5178Y) differing in their double strand breaks and nucleotide excision repair ability. cis-Diaminedichloroplatinum (CDDP) was used as a reference compound. The toxicity of Pt(C7H16N2O2)Cl2 appeared to be similar for both cell lines: IC50 is 8 μM for L5178Y-R cells and 12 μM for L5178Y-S cells, respectively. In contrast, the palladium complex was found to be more toxic for the LY-R cells than for the LY-S cells. The cytotoxicity of both compounds was compared with their ability to induce DNA crosslinks, as measured by the modified comet assay. CDDP caused retardation of the DNA migration induced by 2 Gy of the X-irradiation in a dose-dependent manner. The ability of Pd(C7H16N2O2)Cl2 to retard X-ray induced DNA migration was more pronounced than its platinum analogue and CDDP (see Fig. 6). However, this was not reflected in the toxicity of the compound. Such results indicate that these two compounds may cause a different type of DNA damage and/or that the DNA damage caused by the palladium(II) compound was dealt with in a different manner from that induced by the platinum(II) complex.
AB - The aim of this work was to test the cytotoxicity of newly synthesized cis-type complexes of platinum(II) and palladium(II) dichloride with methyl 3,4-diamine-2,3,4,6-tetradeoxy-α-L-lyxohexopyranoside, [M(C7H16 N2O2)Cl2]·H2O, against two mouse lymphoma cell lines (L5178Y) differing in their double strand breaks and nucleotide excision repair ability. cis-Diaminedichloroplatinum (CDDP) was used as a reference compound. The toxicity of Pt(C7H16N2O2)Cl2 appeared to be similar for both cell lines: IC50 is 8 μM for L5178Y-R cells and 12 μM for L5178Y-S cells, respectively. In contrast, the palladium complex was found to be more toxic for the LY-R cells than for the LY-S cells. The cytotoxicity of both compounds was compared with their ability to induce DNA crosslinks, as measured by the modified comet assay. CDDP caused retardation of the DNA migration induced by 2 Gy of the X-irradiation in a dose-dependent manner. The ability of Pd(C7H16N2O2)Cl2 to retard X-ray induced DNA migration was more pronounced than its platinum analogue and CDDP (see Fig. 6). However, this was not reflected in the toxicity of the compound. Such results indicate that these two compounds may cause a different type of DNA damage and/or that the DNA damage caused by the palladium(II) compound was dealt with in a different manner from that induced by the platinum(II) complex.
KW - Cis-platin
KW - Comet assay
KW - Cytotoxicity
KW - DNA crosslinks
KW - L5178Y cells
KW - Single cell gel electrophoresis
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U2 - 10.1002/tcm.10046
DO - 10.1002/tcm.10046
M3 - Article
C2 - 12616592
AN - SCOPUS:0037288309
SN - 0270-3211
SP - 1
EP - 11
JO - Teratogenesis Carcinogenesis and Mutagenesis
JF - Teratogenesis Carcinogenesis and Mutagenesis
IS - SUPPL. 1
ER -