TY - JOUR
T1 - Digital Examination of LYmph node CYtopathology Using the Sydney system (DELYCYUS)
T2 - An international, multi-institutional study
AU - Caputo, Alessandro
AU - Fraggetta, Filippo
AU - Cretella, Pasquale
AU - Cozzolino, Immacolata
AU - Eccher, Albino
AU - Girolami, Ilaria
AU - Marletta, Stefano
AU - Troncone, Giancarlo
AU - Vigliar, Elena
AU - Acanfora, Gennaro
AU - Zarra, Karen Villar
AU - Torres Rivas, Héctor Enrique
AU - Fadda, Guido
AU - Field, Andrew
AU - Katz, Ruth
AU - Vielh, Philippe
AU - Eloy, Catarina
AU - Rajwanshi, Arvind
AU - Gupta, Nalini
AU - Al-Abbadi, Mousa
AU - Bustami, Nadwa
AU - Arar, Tala
AU - Calaminici, Maria
AU - Raine, Juliet I.
AU - Barroca, Helena
AU - Canão, Pedro Amoroso
AU - Ehinger, Mats
AU - Rajabian, Nilofar
AU - Dey, Pranab
AU - Medeiros, L. Jeffrey
AU - El Hussein, Siba
AU - Lin, Oscar
AU - D’Antonio, Antonio
AU - Bode-Lesniewska, Beata
AU - Rossi, Esther Diana
AU - Zeppa, Pio
N1 - Publisher Copyright:
© 2023 The Authors. Cancer Cytopathology published by Wiley Periodicals LLC on behalf of American Cancer Society.
PY - 2023/11
Y1 - 2023/11
N2 - Background: After a series of standardized reporting systems in cytopathology, the Sydney system was recently introduced to address the need for reproducibility and standardization in lymph node cytopathology. Since then, the risk of malignancy for the categories of the Sydney system has been explored by several studies, but no studies have yet examined the interobserver reproducibility of the Sydney system. Methods: The authors assessed interobserver reproducibility of the Sydney system on 85 lymph node fine-needle aspiration cytology cases reviewed by 15 cytopathologists from 12 institutions in eight different countries, resulting in 1275 diagnoses. In total, 186 slides stained with Diff-Quik, Papanicolaou, and immunocytochemistry were scanned. A subset of the cases included clinical data and results from ultrasound examinations, flow cytometry immunophenotyping, and fluorescence in situ hybridization analysis. The study participants assessed the cases digitally using whole-slide images. Results: Overall, the authors observed an almost perfect agreement of cytopathologists with the ground truth (median weighted Cohen κ = 0.887; interquartile range, κ = 0.210) and moderate overall interobserver concordance (Fleiss κ = 0.476). There was substantial agreement for the inadequate and malignant categories (κ = 0.794 and κ = 0.729, respectively), moderate agreement for the benign category (κ = 0.490), and very slight agreement for the suspicious (κ = 0.104) and atypical (κ = 0.075) categories. Conclusions: The Sydney system for reporting lymph node cytopathology shows adequate interobserver concordance. Digital microscopy is an adequate means to assess lymph node cytopathology specimens.
AB - Background: After a series of standardized reporting systems in cytopathology, the Sydney system was recently introduced to address the need for reproducibility and standardization in lymph node cytopathology. Since then, the risk of malignancy for the categories of the Sydney system has been explored by several studies, but no studies have yet examined the interobserver reproducibility of the Sydney system. Methods: The authors assessed interobserver reproducibility of the Sydney system on 85 lymph node fine-needle aspiration cytology cases reviewed by 15 cytopathologists from 12 institutions in eight different countries, resulting in 1275 diagnoses. In total, 186 slides stained with Diff-Quik, Papanicolaou, and immunocytochemistry were scanned. A subset of the cases included clinical data and results from ultrasound examinations, flow cytometry immunophenotyping, and fluorescence in situ hybridization analysis. The study participants assessed the cases digitally using whole-slide images. Results: Overall, the authors observed an almost perfect agreement of cytopathologists with the ground truth (median weighted Cohen κ = 0.887; interquartile range, κ = 0.210) and moderate overall interobserver concordance (Fleiss κ = 0.476). There was substantial agreement for the inadequate and malignant categories (κ = 0.794 and κ = 0.729, respectively), moderate agreement for the benign category (κ = 0.490), and very slight agreement for the suspicious (κ = 0.104) and atypical (κ = 0.075) categories. Conclusions: The Sydney system for reporting lymph node cytopathology shows adequate interobserver concordance. Digital microscopy is an adequate means to assess lymph node cytopathology specimens.
KW - digital cytopathology
KW - lymph node
KW - reproducibility
KW - virtual microscopy
KW - whole-slide imaging
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U2 - 10.1002/cncy.22741
DO - 10.1002/cncy.22741
M3 - Article
C2 - 37418195
AN - SCOPUS:85164133837
SN - 1934-662X
VL - 131
SP - 679
EP - 692
JO - Cancer Cytopathology
JF - Cancer Cytopathology
IS - 11
ER -