TY - JOUR
T1 - Dimethyl sulfoxide enhances effectiveness of skin antiseptics and reduces contamination rates of blood cultures
AU - Tarrand, Jeffrey J.
AU - LaSala, Paul R.
AU - Han, Xiang Yang
AU - Rolston, Kenneth V.
AU - Kontoyiannis, Dimitrios P.
PY - 2012/5
Y1 - 2012/5
N2 - Effective skin antisepsis is of central importance in the prevention of wound infections, colonization of medical devices, and nosocomial transmission of microorganisms. Current antiseptics have a suboptimal efficacy resulting in substantial infectious morbidity, mortality, and increased health care costs. Here, we introduce an in vitro method for antiseptic testing and a novel alcohol-based anti-septic containing 4 to5%of the polar aprotic solvent dimethyl sulfoxide (DMSO). The DMSO-containing antiseptic resulted in a 1-to 2-log enhanced killing of Staphylococcus epidermidis and other microbes in vitro compared to the same antiseptic without DMSO. In a prospective clinical validation, blood culture contamination rates were reduced from 3.04% for 70% isopropanol-1% iodine (control antiseptic) to 1.04% for 70% isopropanol-1% iodine-5% DMSO (P<0.01). Our results predict that improved skin antisepsis is possible using new formulations of antiseptics containing strongly polarized but nonionizing (polar aprotic) solvents.
AB - Effective skin antisepsis is of central importance in the prevention of wound infections, colonization of medical devices, and nosocomial transmission of microorganisms. Current antiseptics have a suboptimal efficacy resulting in substantial infectious morbidity, mortality, and increased health care costs. Here, we introduce an in vitro method for antiseptic testing and a novel alcohol-based anti-septic containing 4 to5%of the polar aprotic solvent dimethyl sulfoxide (DMSO). The DMSO-containing antiseptic resulted in a 1-to 2-log enhanced killing of Staphylococcus epidermidis and other microbes in vitro compared to the same antiseptic without DMSO. In a prospective clinical validation, blood culture contamination rates were reduced from 3.04% for 70% isopropanol-1% iodine (control antiseptic) to 1.04% for 70% isopropanol-1% iodine-5% DMSO (P<0.01). Our results predict that improved skin antisepsis is possible using new formulations of antiseptics containing strongly polarized but nonionizing (polar aprotic) solvents.
UR - http://www.scopus.com/inward/record.url?scp=84860004857&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84860004857&partnerID=8YFLogxK
U2 - 10.1128/JCM.05106-11
DO - 10.1128/JCM.05106-11
M3 - Article
C2 - 22378911
AN - SCOPUS:84860004857
SN - 0095-1137
VL - 50
SP - 1552
EP - 1557
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
IS - 5
ER -