Abstract
A novel high-performance liquid chromatography with chemiluminescence detection has been developed for the determination of trans-resveratrol in red wines based on the enhancement by trans-resveratrol of the chemiluminescence from luminol-potassium ferricyanide system in alkaline medium. Samples were separated on a C8 column using isocratic elution of methanol/water (35:65). Quantification of trans-resveratrol in Chinese red wines was performed without any sample pretreatment. It allows for the determination of trans-resveratrol in the range 0.5-750 μg/L with a detection limit of 0.166 μg/L (S/N=3). The relative standard deviation (RSD) is 1.16% for 7.5 μg/L trans-resveratrol (n=11). trans-Resveratrol was detected in Chinese red wines with the recoveries of 92.2-114.7%. Concentrations ranged from 0.072 to 1.607 mg/L in Chinese red wines.
Original language | English (US) |
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Pages (from-to) | 613-620 |
Number of pages | 8 |
Journal | Food Chemistry |
Volume | 88 |
Issue number | 4 |
DOIs | |
State | Published - Dec 2004 |
Keywords
- Chemiluminescence
- Chinese red wine
- Liquid chromatography
- trans-Resveratrol
ASJC Scopus subject areas
- Analytical Chemistry
- Food Science