TY - JOUR
T1 - Disclosing quantitative RT-PCR raw data during manuscript submission
T2 - a call for action
AU - for the HEROIC (tHe initiativE gRoup On qRT dIsClosure) Consortium
AU - Untergasser, Andreas
AU - Hellemans, Jan
AU - Pfaffl, Michael W.
AU - Ruijter, Jan M.
AU - van den Hoff, Maurice J.B.
AU - Dragomir, Mihnea P.
AU - Adamoski, Douglas
AU - Dias, Sandra Martha Gomes
AU - Reis, Rui Manuel
AU - Ferracin, Manuela
AU - Dias-Neto, Emmanuel
AU - Marsh, Ian
AU - Kubista, Mikael
AU - Fabbri, Muller
AU - Goel, Ajay
AU - Slabý, Ondřej
AU - Knutsen, Erik
AU - Chen, Baoqing
AU - Negrini, Massimo
AU - Mimori, Koshi
AU - Pichler, Martin
AU - Papatriantafyllou, Maria
AU - Anfossi, Simone
AU - Schmittgen, Thomas D.
AU - Huggett, Jim
AU - Bustin, Stephen
AU - Vandesompele, Jo
AU - Calin, George A.
N1 - Publisher Copyright:
© 2023 The Authors. Molecular Oncology published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.
PY - 2023/5
Y1 - 2023/5
N2 - Accuracy and transparency of scientific data are becoming more and more relevant with the increasing concern regarding the evaluation of data reproducibility in many research areas. This concern is also true for quantifying coding and noncoding RNAs, with the remarkable increase in publications reporting RNA profiling and sequencing studies. To address the problem, we propose the following recommendations: (a) accurate documentation of experimental procedures in Materials and methods (and not only in the supplementary information, as many journals have a strict mandate for making Materials and methods as visible as possible in the main text); (b) submission of RT-qPCR raw data for all experiments reported; and (c) adoption of a unified, simple format for submitted RT-qPCR raw data. The Real-time PCR Data Essential Spreadsheet Format (RDES) was created for this purpose.
AB - Accuracy and transparency of scientific data are becoming more and more relevant with the increasing concern regarding the evaluation of data reproducibility in many research areas. This concern is also true for quantifying coding and noncoding RNAs, with the remarkable increase in publications reporting RNA profiling and sequencing studies. To address the problem, we propose the following recommendations: (a) accurate documentation of experimental procedures in Materials and methods (and not only in the supplementary information, as many journals have a strict mandate for making Materials and methods as visible as possible in the main text); (b) submission of RT-qPCR raw data for all experiments reported; and (c) adoption of a unified, simple format for submitted RT-qPCR raw data. The Real-time PCR Data Essential Spreadsheet Format (RDES) was created for this purpose.
KW - RNA
KW - RT-qPCR
KW - accuracy
KW - quantification
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U2 - 10.1002/1878-0261.13418
DO - 10.1002/1878-0261.13418
M3 - Article
C2 - 36916500
AN - SCOPUS:85151090211
SN - 1574-7891
VL - 17
SP - 713
EP - 717
JO - Molecular oncology
JF - Molecular oncology
IS - 5
ER -