Abstract
Stimulated lymphocytes which pass through the cell cycle may be distinguished from dormant G0 lymphocytes rapidly by flow cytometry. The method is based on cell incubation with 5-bromodeoxyuridine (BUdR) and their subsequent staining with acridine orange under conditions in which cellular DNA and RNA stain differentially. The DNA-specific green fluorescence of stimulated, cycling cells is suppressed while RNA-specific red fluorescence is affected only minimally. It is possible, therefore, to distinguish cycling vs non-cycling cells based on two entirely different parameters, i.e. BUdR incorporation and RNA content.
Original language | English (US) |
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Pages (from-to) | 31-35 |
Number of pages | 5 |
Journal | Experimental Cell Research |
Volume | 115 |
Issue number | 1 |
DOIs | |
State | Published - Aug 1978 |
Externally published | Yes |
ASJC Scopus subject areas
- Cell Biology